Overview
- Presents detailed, thorough protocols examining this burgeoning field of study
- Features techniques easily adaptable to numerous research tasks
- Contains expert notes and vital implementation advice
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 717)
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Table of contents (17 protocols)
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Front Matter
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Antibodies as a Tool: From Concept to Design and Application
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Front Matter
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Preservation and Unmasking of Tissue Antigens
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Front Matter
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Imaging Techniques and High-Throughput Data Analysis
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Front Matter
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Imaging Techniqu es and High-Throughput Data Analysis
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Neuroscience, Cancer, and Stem Cell Research
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Front Matter
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Novel Assays and Techniques
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Front Matter
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Keywords
About this book
Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.
Reviews
From the reviews:
“Each of us daily using immunohistochemical protocols to reveal targets either useful for research or diagnostic aims will surely wonder by which tricky techniques it is possible to overcome the preservation and unmasking of those labile antigens involved in signal transduction. Well, by seventheen chapters grouped in five parts Prof. Alexander E. Kalyuzhny is presenting an invaluable technical and methodological source of hints to satisfy our needs: to overcome troubleshottings if we are already in the field or to orientate those entering the field.” (Carlo Albert Redi, European Journal of Histochemistry, July, 2011)Editors and Affiliations
Bibliographic Information
Book Title: Signal Transduction Immunohistochemistry
Book Subtitle: Methods and Protocols
Editors: Alexander E. Kalyuzhny
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-024-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-023-2Published: 14 February 2011
Softcover ISBN: 978-1-4939-5804-7Published: 23 August 2016
eBook ISBN: 978-1-61779-024-9Published: 03 March 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 302
Topics: Biochemistry, general, Antibodies