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Regulatory T Cells

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. George Kassiotis

    1. , Division of Immunoregulation, MRC National Institute for Medical Resea, London, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Adrian Liston

    1. , VIB Autoimmune Genetics Laboratory, Katholieke Universiteit Leuven, Leuven, Belgium

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides optimized and standardized protocols to allow reliable and reproducible use
  • Presents innovations that have lead to insights on the suppressive mechanisms and biology of regulatory T cells
  • Features tips from the experts to ensure the flawless implementation of the techniques
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 707)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Regulatory T Cells: History and Perspective

      • Shimon Sakaguchi
      Pages 3-17

  3. In Vitro

    1. Front Matter

      Pages 19-19
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    2. In Vitro Treg Suppression Assays

      • Lauren W. Collison, Dario A. A. Vignali
      Pages 21-37

    3. Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells

      • Nagendra Singh, Rafal Pacholczyk, Makio Iwashima, Leszek Ignatowicz
      Pages 39-44

    4. In Vitro and In Vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells

      • Kim J. Hasenkrug, Lara M. Myers
      Pages 45-54

    5. Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus

      • Donald M. Simons, Andrew J. Caton
      Pages 55-69

    6. ChIP-on-Chip for FoxP3

      • Ye Zheng
      Pages 71-82

    7. Live Imaging of Dendritic Cell–Treg Cell Interactions

      • Milka Sarris, Alexander G. Betz
      Pages 83-101

  4. In Vivo

    1. Front Matter

      Pages 103-103
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    2. Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In Vivo

      • Nadia M. Jeremiah, Adrian Liston
      Pages 105-118

    3. In Vivo Treg Suppression Assays

      • Creg J. Workman, Lauren W. Collison, Maria Bettini, Meenu R. Pillai, Jerold E. Rehg, Dario A. A. Vignali
      Pages 119-156

    4. In Vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model

      • Katharina Lahl, Tim Sparwasser
      Pages 157-172

    5. Antigen-Specific Induction of Regulatory T Cells In Vivo and In Vitro

      • Carolin Daniel, Hidde Ploegh, Harald von Boehmer
      Pages 173-185

    6. In Vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection

      • Clémence Nouzé, Lise Pasquet, Joost P. M. van Meerwijk
      Pages 187-196

  5. Human

    1. Front Matter

      Pages 197-197
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    2. Analysis of Human FOXP3+ Treg Cells Phenotype and Function

      • Eva d’Hennezel, Ciriaco A. Piccirillo
      Pages 199-218

    3. Depletion of Human Regulatory T Cells

      • Amy C. Hobeika, Michael A. Morse, Takuya Osada, Sharon Peplinski, H. Kim Lyerly, Timothy M. Clay
      Pages 219-231

    4. Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In Vitro Assay

      • Anya Schneider, Jane H. Buckner
      Pages 233-241
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Keywords

About this book

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.

Reviews

From the reviews:

“This book is to provide a detailed and up-to-date collection of methods and protocols utilised for the analysis of regulatory T cells. … this book would be of most use to scientific researchers, in particular PhD students and post-doctoral researchers, working in the field of regulatory T cells within a laboratory setting. For individuals using in vitro or in vivo mouse models for regulatory T cell analysis, this book would be a perfect asset.” (Karen Smith, Immunology News, February, 2012)

“This volume in the Methods in Molecular Biology series is a collection of current techniques to study regulatory T cells. … Researchers who need to discover techniques to isolate or use regulatory T cells are the primary audience. The authors of the individual chapters are knowledgeable about the techniques they describe. … There are other books that discuss regulatory T cells, but not with the level of procedural detail that this one has. It would be a useful reference for a library … .” (Marion C. Cohen, Doody's Book Reviews, August, 2011)

Editors and Affiliations

  • , Division of Immunoregulation, MRC National Institute for Medical Resea, London, United Kingdom

    George Kassiotis

  • , VIB Autoimmune Genetics Laboratory, Katholieke Universiteit Leuven, Leuven, Belgium

    Adrian Liston

Bibliographic Information

  • Book Title: Regulatory T Cells

  • Book Subtitle: Methods and Protocols

  • Editors: George Kassiotis, Adrian Liston

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61737-979-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61737-978-9Published: 09 February 2011

  • Softcover ISBN: 978-1-4939-6158-0Published: 23 August 2016

  • eBook ISBN: 978-1-61737-979-6Published: 03 February 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Topics: Immunology, Cell Culture

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