Overview
- Editors:
-
-
Thomas C. Evans, Jr.
-
, DNA Enzymes Division, New England Biolabs, Inc., Ipswich, USA
-
Ming-Qun Xu
-
, Chemical Biology Division, New England Biolabs, Inc., Ipswich, USA
- Helps to guide scientists toward an ideal expression host
- Provides easy to follow protocols, ready to be implemented in the lab
- Includes tips from the experienced, expert contributors on troubleshooting common methodological pitfalls
- Includes supplementary material: sn.pub/extras
Access this book
Other ways to access
Table of contents (18 protocols)
-
-
- Evelina Angov, Patricia M. Legler, Ryan M. Mease
Pages 1-13
-
- Raymond J. Peroutka III, Steven J. Orcutt, James E. Strickler, Tauseef R. Butt
Pages 15-30
-
-
- Adam C. Fisher, Mark A. Rocco, Matthew P. DeLisa
Pages 53-67
-
-
- Inca Ghosh, Nancy Considine, Elissa Maunus, Luo Sun, Aihua Zhang, John Buswell et al.
Pages 87-107
-
-
-
- Jonathan P. Richardson, Derek Macmillan
Pages 151-174
-
- Orsolya Király, Lan Guan, Miklós Sahin-Tóth
Pages 175-194
-
-
- Martin Schlapschy, Arne Skerra
Pages 211-224
-
- Jun-ichi Nagao, Kouki Shioya, Yoshitaka Harada, Ken-ichi Okuda, Takeshi Zendo, Jiro Nakayama et al.
Pages 225-236
-
- Joe Tiralongo, Andrea Maggioni
Pages 237-249
-
- Akira Kanno, Takeaki Ozawa, Yoshio Umezawa
Pages 251-258
-
- Ping Sun, Joseph E. Tropea, David S. Waugh
Pages 259-274
-
-
- Aihua Zhang, Luo Sun, John Buswell, Nancy Considine, Inca Ghosh, Anastasiya Masharina et al.
Pages 295-307
-
Back Matter
Pages 309-310
About this book
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Reviews
From the reviews:
“The editors bring together a diverse set of methods and examples demonstrating that, in fact, there is still plenty of active and exiting research pushing back some of the aforementioned limitations of E. coli. … If recombinant protein expression is an important part of your research programme, either in an academic or industrial setting, then this book is worth a read – it could save you a fortune!” (Gavin Thomas, Microbiology Today, November, 2011)
Editors and Affiliations
-
, DNA Enzymes Division, New England Biolabs, Inc., Ipswich, USA
Thomas C. Evans, Jr.
-
, Chemical Biology Division, New England Biolabs, Inc., Ipswich, USA
Ming-Qun Xu
