Overview
- Editors:
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James A. Birchler
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, Division of Biological Sciences, University of Missouri, Columbia, USA
- Focuses on vital techniques which can lead to the production of improved food, feed, fuel, fiber, and pharmaceuticals
- Contains protocols tested and proven by expert researchers in the field
- Features the product of the contributors experience in the form of key tips for the successful implementation of the methods
- Includes supplementary material: sn.pub/extras
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Table of contents (18 protocols)
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- Shyamkumar Barampuram, Zhanyuan J. Zhang
Pages 1-35
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- Michal Lieberman-Lazarovich, Avraham A. Levy
Pages 51-65
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- Akio Kato, Jonathan C. Lamb, Patrice S. Albert, Tatiana Danilova, Fangpu Han, Zhi Gao et al.
Pages 67-96
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- Andreas Houben, Shuhei Nasuda, Takashi R. Endo
Pages 97-111
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- Robert T. Gaeta, Lakshminarasimhan Krishnaswamy
Pages 131-146
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- Yuan-Yeu Yau, Yueju Wang, James G. Thomson, David W. Ow
Pages 147-166
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- Feng Zhang, Daniel F. Voytas
Pages 167-177
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- Andrew L. Eamens, Peter M. Waterhouse
Pages 179-197
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- Nobuyuki Matoba, Keith R. Davis, Kenneth E. Palmer
Pages 199-219
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- Jaroslav Doležel, Marie Kubaláková, Jarmila ÃhalÃková, Pavla Suchánková, Hana Å imková
Pages 221-238
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- Dal-Hoe Koo, Jiming Jiang
Pages 239-245
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- Ralf G. Kynast, Oscar Riera-Lizarazu
Pages 259-284
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- Aniça Amini, Chongyuan Luo, Eric Lam
Pages 301-314
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- Jianbo Zhang, Chuanhe Yu, Lakshminarasimhan Krishnaswamy, Thomas Peterson
Pages 315-326
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Back Matter
Pages 327-329
About this book
With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biologyâ„¢ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.
Editors and Affiliations
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, Division of Biological Sciences, University of Missouri, Columbia, USA
James A. Birchler