Alzheimer's Disease and Frontotemporal Dementia

1. Front Matter

   Pages i-x

   PDF

2. Working with AD- and FTD-Related Molecules

   1. Front Matter

      Pages 11-11

      PDF

3. Contemporary Approaches to Alzheimer’s Disease and Frontotemporal Dementia

   1. Contemporary Approaches to Alzheimer’s Disease and Frontotemporal Dementia

      • Erik D. Roberson

      Pages 1-9

4. Working with AD- and FTD-Related Molecules

   1. Front Matter

      Pages 11-11

      PDF

   2. Preparing Synthetic Aβ in Different Aggregation States

      • W. Blaine Stine, Lisa Jungbauer, Chunjiang Yu, Mary Jo LaDu

      Pages 13-32

   3. Isolation of Low-n Amyloid β-Protein Oligomers from Cultured Cells, CSF, and Brain

      • Ganesh M. Shankar, Alfred T. Welzel, Jessica M. McDonald, Dennis J. Selkoe, Dominic M. Walsh

      Pages 33-44

   4. Detecting Aβ*56 Oligomers in Brain Tissues

      • Mathew A. Sherman, Sylvain E. Lesné

      Pages 45-56

   5. Assessing Aβ Aggregation State by Atomic Force Microscopy

      • Justin Legleiter

      Pages 57-70

   6. Measuring APP Carboxy-Terminal Fragments

      • Luke A. Esposito

      Pages 71-84

   7. Detection of APP Intracellular Domain in Brain Tissue

      • Sanjay W. Pimplikar, Anupama Suryanarayana

      Pages 85-91

   8. Cell-Based Assays for Regulators of Tau Biology

      • Umesh K. Jinwal, Chad A. Dickey

      Pages 93-108

   9. Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In Situ

      • Wanjoo Chun, Geoffrey S. Waldo, Gail V. W. Johnson

      Pages 109-123

   10. Apolipoprotein E Expression and Purification

       • Yvonne Newhouse, Karl H. Weisgraber

       Pages 127-140

5. Model Systems and Outcome Measures

   1. Front Matter

      Pages 125-125

      PDF

   2. Aβ Toxicity in Primary Cultured Neurons

      • Adriana Ferreira, Roxana C. Sinjoanu, Alexandra Nicholson, Sara Kleinschmidt

      Pages 141-153

   3. Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors

      • Binggui Sun, Li Gan

      Pages 155-168

   4. Selecting a Mouse Model of Alzheimer’s Disease

      • Jeannie Chin

      Pages 169-189

   5. Monitoring Spatial Learning and Memory in Alzheimer’s Disease Mouse Models Using the Morris Water Maze

      • Kimberly Scearce-Levie

      Pages 191-205

   6. Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain

      • Jorge J. Palop, Erik D. Roberson, Inma Cobos

      Pages 207-230

   7. Real-Time Visualization of Axonal Transport in Neurons

      • Yasuko Osakada, Bianxiao Cui

      Pages 231-243

Alzheimer’s disease and frontotemporal dementia, two of the most prevalent neurodegenerative diseases, are dreaded disorders that attack the neural networks underlying memory and personality, systems that make us who we are. Major breakthroughs in recent years have spurred new research approaches to these conditions. In Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols, expert investigators bring together the many divergent areas of expertise used to study these diseases, including behavior, electrophysiology, confocal microscopy, and hardcore biochemistry. Beginning with an overview of the two diseases and contemporary research on them, the book continues with specialized protocols for working with amyloid-β peptide, tau, and apolipoprotein E as well as experimental systems for studying AD and FTD, including cell and animal models, and outcome measures that can be used to assess neuronal function in these systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols serves as an ideal guide for all scientists pursuing the cause and cure of these widespread and nightmarishly debilitating disorders.

• Amyloid-β peptide & apolipoprotein E
• Cell and animal models
• Genetic risk factors
• Molecular neuropathology
• Neurodegenerative disorder
• Neuronal dysfunction
• Pathobiochemistry

From the reviews:

“This book brings together research authorities in the field of Alzheimer’s disease (AD) and frontotemporal dementia (FTD) to share their laboratory methods and protocols for molecular and model systems and outcome research in the field of neurodegenerative biomedical research. … The audience includes all laboratory scientists interested in molecular and neuroscience research that seeks a cause and cure for these two devastating disorders. … The book is thoughtfully written and will have the greatest appeal for those with a familiarity with laboratory research methods.” (Charlene Hoffman Snyder, Doody’s Review Service, February, 2011)

“This is a collection of relatively modern methodologies, focused primarily on the study of amyloid-β processing … . Research technicians will find the ‘notes’ sections particularly valuable … . provides a nice reference for anyone interested in studying putative models of Alzheimer’s disease. … Overall, the book is well organized, well written, appropriately succinct, and exceedingly useful in those areas that are addressed, and provides a valuable reference to the basic scientist studying Alzheimer’s disease.” (Rudy J. Castellani, Journal of Alzheimer’s Disease, Vol. 25, 2011)

• Ctr. Neurodegeneration & Experimental Th, Depts. of Neurology and Neurobiology, University of Alabama at Birmingham, Birmingham, USA

  Erik D. Roberson

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