Overview
- Provides the latest protocols to study the mechanical properties of the nucleus and for imaging down to the molecular level by optical, atomic force, and electron microscopy
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 464)
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Table of contents (24 protocols)
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Front Matter
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Physical and Mechanical Properties
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Front Matter
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Chromatin and Transcription
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Front Matter
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Keywords
About this book
Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging presents biophysical approaches to study the mechanical properties of nuclei, together with a comprehensive range of imaging methods. These include FISH, FRAP, FRET, molecular beacons, fluorescence correlation spectroscopy, single molecule tracking, and combing DNA for optical microscopy, recognition imaging for atomic force microscopy, and hybridisation, tomography, and spectroscopic imaging for electron microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.
The Nucleus, Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today.
Editors and Affiliations
Bibliographic Information
Book Title: The Nucleus
Book Subtitle: Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging
Editors: Ronald Hancock
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-461-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-60327-460-9Published: 28 August 2008
Softcover ISBN: 978-1-61737-898-0Published: 10 December 2010
eBook ISBN: 978-1-60327-461-6Published: 27 October 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 444
Number of Illustrations: 82 b/w illustrations, 14 illustrations in colour
Topics: Biochemistry, general, Cell Biology, Cell Physiology