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Protein Folding, Misfolding, and Disease

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Andrew F. Hill

    1. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Parkville, Australia

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  2. Kevin J. Barnham

    1. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Parkville, Australia

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  3. Stephen P. Bottomley

    1. , Department of Biochemistry &, Monash University, Clayton, Australia

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  4. Roberto Cappai

    1. Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Parkville, Australia

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  • Brings together a thorough collection of cutting-edge methods for protein folding and misfolding
  • Features concise, step-by-step approaches to the varying topics
  • Includes key tips and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 752)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Strategies for Boosting the Accumulation of Correctly Folded Recombinant Proteins Expressed in Escherichia coli

    • Ario de Marco
    Pages 1-15

  3. An Escherichia coli Cell-Free System for Recombinant Protein Synthesis on a Milligram Scale

    • Luke A. Miles, Gabriela A. N. Crespi, Sen Han, Andrew F. Hill, Michael W. Parker
    Pages 17-28

  4. Synthesis of Peptide Sequences Derived from Fibril-Forming Proteins

    • Denis B. Scanlon, John A. Karas
    Pages 29-43

  5. Refolding Your Protein with a Little Help from REFOLD

    • Jennifer Phan, Nasrin Yamout, Jason Schmidberger, Stephen P. Bottomley, Ashley M. Buckle
    Pages 45-57

  6. Circular Dichroism and Its Use in Protein-Folding Studies

    • David T. Clarke
    Pages 59-72

  7. Distance Measurements by Continuous Wave EPR Spectroscopy to Monitor Protein Folding

    • James A. Cooke, Louise J. Brown
    Pages 73-96

  8. Solution-State Nuclear Magnetic Resonance Spectroscopy and Protein Folding

    • Lisa D. Cabrita, Christopher A. Waudby, Christopher M. Dobson, John Christodoulou
    Pages 97-120

  9. Diagnostics for Amyloid Fibril Formation: Where to Begin?

    • Danny M. Hatters, Michael D. W. Griffin
    Pages 121-136

  10. Probing Protein Aggregation with Quartz Crystal Microbalances

    • Tuomas P. J. Knowles, Glyn L. Devlin, Christopher M. Dobson, Mark E. Welland
    Pages 137-145

  11. Dried and Hydrated X-Ray Scattering Analysis of Amyloid Fibrils

    • Sally L. Gras, Adam M. Squires
    Pages 147-163

  12. Solid-State NMR of Amyloid Membrane Interactions

    • John D. Gehman, Frances Separovic
    Pages 165-177

  13. Sedimentation Velocity Analysis of Amyloid Fibrils

    • Chi Le Lan Pham, Yee-Foong Mok, Geoffrey J. Howlett
    Pages 179-196

  14. Transmission Electron Microscopy of Amyloid Fibrils

    • Sally L. Gras, Lynne J. Waddington, Kenneth N. Goldie
    Pages 197-214

  15. Surface Plasmon Resonance Spectroscopy: A New Lead in Studying the Membrane Binding of Amyloidogenic Transthyretin

    • Xu Hou, David H. Small, Marie-Isabel Aguilar
    Pages 215-228

  16. Elucidating the Role of Metals in Alzheimer’s Disease Through the Use of Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry

    • Andrew D. Watt, Keyla A. Perez, Lin Wai Hung
    Pages 229-240

  17. Back Matter

    Pages 241-249
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Keywords

About this book

Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease.  In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of –the-art technologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Up to date and authoritative, Protein Folding, Misfolding, and Disease: Methods and Protocols offers researchers the tools necessary to move ahead in this vital field.

Editors and Affiliations

  • Bio21 Molecular Science and, Biotechnology Institute, University of Melbourne, Parkville, Australia

    Andrew F. Hill, Kevin J. Barnham, Roberto Cappai

  • , Department of Biochemistry &, Monash University, Clayton, Australia

    Stephen P. Bottomley

Bibliographic Information

  • Book Title: Protein Folding, Misfolding, and Disease

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew F. Hill, Kevin J. Barnham, Stephen P. Bottomley, Roberto Cappai

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-223-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-60327-221-6Published: 01 July 2011

  • Softcover ISBN: 978-1-4939-5689-0Published: 23 August 2016

  • eBook ISBN: 978-1-60327-223-0Published: 29 June 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 249

  • Topics: Protein Science, Pathology

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