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Mesenchymal Stem Cells

Methods and Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Darwin J. Prockop

    1. Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA

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    You can also search for this editor in PubMed   Google Scholar

  2. Bruce A. Bunnell

    1. Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA

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    You can also search for this editor in PubMed   Google Scholar

  3. Donald G. Phinney

    1. Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA

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    You can also search for this editor in PubMed   Google Scholar

  • An essential guide to MSC research for both newcomers and established researchers in the field
  • Contains chapters on isolation and expansion of mouse and human stem cells
  • Explores stem cells from bone marrow and adipose tissue
  • Provides procedures for differentiation of stem cells along multiple lineages
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 449)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Isolation of Human Multipotential Stromal Cells from Bone Marrow and Adipose Tissue

    1. Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)

      • Margaret Wolfe, Radhika Pochampally, William Swaney, Roxanne L. Reger
      Pages 3-25

    2. Mesenchymal Stem Cells from Adult Bone Marrow

      • Mark F. Pittenger
      Pages 27-44

    3. A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells

      • Stan Gronthos, Andrew C. W. Zannettino
      Pages 45-57

    4. Adipose-Derived Stem Cells

      • John K. Fraser, Min Zhu, Isabella Wulur, Zeni Alfonso
      Pages 59-67

    5. Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens

      • Severine G. Dubois, Elizabeth Z. Floyd, Sanjin Zvonic, Gail Kilroy, Xiying Wu, Stacy Carling et al.
      Pages 69-79

  3. Manipulation of Human Multipotential Stromal Cells

    1. Colony Forming Unit Assays for MSCs

      • Radhika Pochampally
      Pages 83-91

    2. Differentiation and Characterization of Human MSCs

      • Roxanne L. Reger, Alan H. Tucker, Margaret R. Wolfe
      Pages 93-107

    3. Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis, and Experimentation

      • Roxanne L. Reger, Margaret R. Wolfe
      Pages 109-116

    4. Gene Expression Analysis at the Single Cell Level Using the Human Bone Marrow Stromal Cell as a Model: Sample Preparation Methods

      • Beerelli Seshi
      Pages 117-132

    5. Assays of MSCs with Microarrays

      • Joni YlÅ‘stalo, Radhika Pochampally, Darwin J. Prockop
      Pages 133-151

    6. Gene Delivery to Mesenchymal Stem Cells

      • Reza Izadpanah, Bruce A. Bunnell
      Pages 153-167

  4. Isolation and Characterization of Murine Multipotential Stromal Cells from Bone Marrow

    1. Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion

      • Donald G. Phinney
      Pages 171-186

  5. Back Matter

    Pages 187-192
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Keywords

About this book

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study.

Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.

Editors and Affiliations

  • Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA

    Darwin J. Prockop, Bruce A. Bunnell, Donald G. Phinney

Bibliographic Information

  • Book Title: Mesenchymal Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Darwin J. Prockop, Bruce A. Bunnell, Donald G. Phinney

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-169-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-771-6Published: 07 March 2008

  • Softcover ISBN: 978-1-61737-767-9Published: 01 December 2010

  • eBook ISBN: 978-1-60327-169-1Published: 15 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 192

  • Topics: Cell Biology, Molecular Medicine, Immunology

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