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Lipid Signaling Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Banafshé Larijani

    1. Dept. Cell Biophysics, Cancer Research UK, London, United Kingdom

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  2. Rudiger Woscholski

    1. Fac. Natural Sciences, Div. Cell & Molecular Biology, Imperial College London, London, United Kingdom

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  3. Colin A. Rosser

    1. Rye St. Antony School, Oxford, United Kingdom

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  • Describes cutting-edge lipid analysis and quantification methods
  • Provides a selection of practical approaches to the study of lipid metabolism and recognition in the cellular context
  • Includes reproducible, step-by-step laboratory protocols
  • Chapters written by carefully selected experts in the field

Part of the book series: Methods in Molecular Biology (MIMB, volume 462)

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Table of contents (26 protocols)

  1. Front Matter

    Pages 1-18
    Download chapter PDF

  2. Lipid Systems and Techniques

    1. Front Matter

      Pages 1-3
      Download chapter PDF

    2. Eicosanoids: Generation and Detection in Mammalian Cells

      • Valerie B. O’Donnell, Ben Maskrey, Graham W. Taylor
      Pages 1-19

    3. Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry

      • Trevor R. Pettitt
      Pages 1-17

    4. Measurement of Polyphosphoinositides in Cultured Mammalian Cells

      • Frank T. Cooke
      Pages 1-16

    5. Inositol Lipid-Dependent Functions in Saccharomyces cerevisiae: Analysis of Phosphatidylinositol Phosphates

      • Stephen K. Dove, Robert H. Michell
      Pages 1-16

    6. Methods for the Determination of the Mass of Nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P 2

      • David R. Jones, Yvette Bultsma, Willem Jan Keune, Nullin Divecha
      Pages 1-14

    7. Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin

      • Marie Garnier-Lhomme, Erick J. Dufourc, Banafshé Larijani, Dominic Poccia
      Pages 1-22

    8. Solution and Solid-State NMR of Lipids

      • Axelle Grelard, Anthony Couvreux, Cécile Loudet, Erick J. Dufourc
      Pages 1-23

    9. Spatial Localization of PtdInsP 2 in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain

      • Xavier Mulet, Erika Rosivatz, Ka Kei Ho, Béatrice L.L.E. Gauthé, Oscar Ces, Richard H. Templer et al.
      Pages 1-10

    10. Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes

      • Sebastian J. Fleire, Facundo D. Batista
      Pages 1-10

    11. Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes

      • F.-Xabier Contreras, Ana-Victoria Villar, Alicia Alonso, Félix M. Goñi
      Pages 1-11

    12. Effects of Sterols on the Development and Aging of Caenorhabditis elegans

      • Eun-Young Lee, Pan-Young Jeong, Sun-Young Kim, Yhong-Hee Shim, David J. Chitwood, Young-Ki Paik
      Pages 1-13

    13. Proteomic Analysis of the Sterol-Mediated Signaling Pathway in Caenorhabditis elegans

      • Byung-Kwon Choi, Yun-Kyung Shin, Eun-Young Lee, Pan-Young Jeong, Yhong-Hee Shim, David J. Chitwood et al.
      Pages 1-15

  3. Lipid Metabolism and Recognition

    1. Front Matter

      Pages 1-3
      Download chapter PDF

    2. Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt

      • Abdelkader Essafi, Ana R. Gomes, Karen M. Pomeranz, Aleksandra K. Zwolinska, Rana Varshochi, Ursula B. McGovern et al.
      Pages 1-11

    3. Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt

      • Erika Rosivatz, Rudiger Woscholski
      Pages 1-10

    4. Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by in vitro and in vivo Assays

      • Lisa M. Ooms, Jennifer M. Dyson, Anne M. Kong, Christina A. Mitchell
      Pages 1-17

    5. Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic β-Cells and Insulin-Responsive Muscle Cells and Adipocytes

      • Rosanna Cazzolli, Ping Huang, Shuzhi Teng, William E Hughes
      Pages 1-11

    6. Protein Kinase C as an Effector of Lipid-Derived Second Messengers

      • Marie Hélène Paclet, Jan K. Davidson-Moncada, Guillermo López-Lluch, Dongmin Shao, Lodewijk V. Dekker
      Pages 1-11
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Keywords

About this book

Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls.

Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology.

Editors and Affiliations

  • Dept. Cell Biophysics, Cancer Research UK, London, United Kingdom

    Banafshé Larijani

  • Fac. Natural Sciences, Div. Cell & Molecular Biology, Imperial College London, London, United Kingdom

    Rudiger Woscholski

  • Rye St. Antony School, Oxford, United Kingdom

    Colin A. Rosser

Bibliographic Information

  • Book Title: Lipid Signaling Protocols

  • Editors: Banafshé Larijani, Rudiger Woscholski, Colin A. Rosser

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-115-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-727-3Published: 11 November 2008

  • Softcover ISBN: 978-1-62703-882-9Published: 30 October 2014

  • eBook ISBN: 978-1-60327-115-8Published: 28 February 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 429

  • Topics: Lipidology, Cell Biology

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