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  • Book
  • © 2007

Vascular Biology Protocols

  • Includes new updates on traditional methods
  • Provides various methodologies for gene therapy and gene-knockout
  • Highly specific, easy to follow step-by-step laboratory protocols

Part of the book series: Methods in Molecular Medicine (MIMM, volume 139)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. Rat Carotid Artery Balloon Injury Model

    • David A. Tulis
    Pages 1-30
  3. Plaque Rupture Model in Mice

    • Takeshi Sasaki, Kae Nakamura, Masafumi Kuzuya
    Pages 67-75
  4. Immunostaining of Mouse Atherosclerotic Lesions

    • Hong Lu, Debra L. Rateri, Alan Daugherty
    Pages 77-94
  5. Surgical Animal Model of Ventricular Hypertrophy

    • Giuseppe Marano, Alberto U. Ferrari
    Pages 95-104
  6. Animal Models of Hypertension

    • Brett M. Mitchell, Thomas Wallerath, Ulrich Förstermann
    Pages 105-111
  7. Rat Models of Cardiac Insulin Resistance

    • Sanjoy Ghosh, Brian Rodrigues, Jun Ren
    Pages 113-143
  8. The Isolated, Perfused Pseudo-Working Heart Model

    • Gary J. Grover, Rajni Singh
    Pages 145-150
  9. Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice

    • Rajakumar V. Donthi, Paul N. Epstein
    Pages 151-161
  10. Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress

    • Camila Manrique, Guido Lastra, Javad Habibi, Yongzhong Wei, E. Matthew Morris, Craig S. Stump et al.
    Pages 163-179
  11. Detection and Quantification of Apoptosis in the Vasculature

    • Wendy A. Boivin, David J. Granville
    Pages 181-195
  12. Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction

    • Keith R. Brunt, Sean R. R. Hall, Christopher A. Ward, Luis G. Melo
    Pages 197-210
  13. Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells

    • Christopher J. Boos, Andrew D. Blann, Gregory Y. H. Lip
    Pages 211-224
  14. Haptotaxis of Endothelial Cell Migration Under Flow

    • Steve Hsu, Rahul Thakar, Song Li
    Pages 237-250
  15. Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells

    • Nair Sreejayan, Xiaoping Yang
    Pages 283-292

About this book

Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive “manual” in a field that has become as broad and deep as cardiovascular medicine, this volume of “Methods in Molecular Medicine” covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.

Reviews

From the reviews:

"This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models. … Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. … This would be a valuable addition to any laboratory doing research in vascular biology." (Vera P. Shively, Doody’s Review Service, December, 2008)

Editors and Affiliations

  • School of Pharmacy, University of Wyoming, Laramie, USA

    Nair Sreejayan, Jun Ren

Bibliographic Information

  • Book Title: Vascular Biology Protocols

  • Editors: Nair Sreejayan, Jun Ren

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1007/978-1-59745-571-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-574-3Published: 25 July 2008

  • Softcover ISBN: 978-1-61737-650-4Published: 14 December 2011

  • eBook ISBN: 978-1-59745-571-8Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XII, 372

  • Topics: Molecular Medicine, Cardiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access