Overview
- Includes 52 different protein transfer and detection techniques
- Gives a brief overview of 35 additional protein transfer and detection methodologies
- Provides a history of protein transfer development and an overview of membrane proteomics
- Outlines inexpensive protein blotting and detection protocols useful for large college courses
- Allows Investigators to rapidly implement a technique that is new to them and to their laboratories
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 536)
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Table of contents (57 protocols)
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Front Matter
Keywords
About this book
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols", expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
Reviews
From the reviews:
"This informative, hands-on-guide is essential for all investigators interested in learning techniques based on Western blotting which they can easily transfer to their laboratories. … The book is intended for any investigator interested in learning a new technique based on Western blotting. … this hands-on guide will be quite useful and helpful for both investigators and students working in these areas." (Omer Iqbal, Doody’s Review Service, October, 2009)
Editors and Affiliations
Bibliographic Information
Book Title: Protein Blotting and Detection
Book Subtitle: Methods and Protocols
Editors: Biji T. Kurien, R. Hal Scofield
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-542-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-73-2Published: 15 May 2009
Softcover ISBN: 978-1-61737-943-7Published: 26 January 2011
eBook ISBN: 978-1-59745-542-8Published: 20 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 588
Number of Illustrations: 135 b/w illustrations, 6 illustrations in colour
Topics: Protein Science, Proteomics