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Cancer Genomics and Proteomics

Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Paul B. Fisher

    1. Department of Pathology, Columbia University Medical Center, College of Physicians and Surgeons, New York
      Department of Neurosurgery, Columbia University Medical Center, College of Physicians and Surgeons, New York
      Department of Urology, Columbia University Medical Center, College of Physicians and Surgeons, New York

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  • A compendium of techniques and applications in gene identification and function
  • Approaches described in detail are state-of-the art and can be tailored to individual ongoing or planned research projects

Part of the book series: Methods in Molecular Biology (MIMB, volume 383)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Reciprocal Subtraction Differential RNA Display (RSDD)

    • Devanand Sarkar, Dong-chul Kang, Paul B. Fisher
    Pages 1-14

  3. Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)

    • Habib Boukerche, Zao-zhong Su, Dong-chul Kang, Paul B. Fisher
    Pages 15-29

  4. The Application of Differential Display as a Gene Profiling Tool

    • Ken Chien-Neng Chang, Barry Komm, Nichole Bayer Arnold, Murray Korc
    Pages 31-40

  5. Serial Analysis of Gene Expression (SAGE)

    • Fred van Ruissen, Frank Baas
    Pages 41-66

  6. Gene Expression Profile Analysis of Tumors

    • Katia Basso, Riccardo Dalla-Favera
    Pages 67-79

  7. cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-κB Related Genes in Squamous Cell Carcinoma

    • Zhong Chen, Tin-Lap Lee, Xin-Ping Yang, Gang Dong, Amy Loercher, Carter Van Waes
    Pages 81-99

  8. Gene Profiling Uncovers Retinoid Target Genes

    • Yan Ma, Qing Feng, Ian Pitha-Rowe, Sutisak Kitareewan, Ethan Dmitrovsky
    Pages 101-121

  9. Complete Open Reading Frame (C-ORF) Technique

    • Dong-chul Kang, Paul B. Fisher
    Pages 123-133

  10. Chromatin Immunoprecipitation Assays

    • Piyali Dasgupta, Srikumar P. Chellappan
    Pages 135-152

  11. Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection

    • Stephanie F. Phelps, Sharon Illenye, Nicholas H. Heintz
    Pages 153-163

  12. Molecular Cytogenetic Applications in Analysis of the Cancer Genome

    • Pulivarthi H. Rao, Subhadra V. Nandula, Vundavalli V. Murty
    Pages 165-185

  13. Monitoring Methylation and Gene Expression in Cancer

    • Hetty Carraway, James Herman
    Pages 187-202

  14. The Use of Phage Display Peptide Libraries for Basic and Translational Research

    • Renee Brissette, Neil I. Goldstein
    Pages 203-213

  15. Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions

    • Shu-ichi Matsuzawa, John C. Reed
    Pages 215-225

  16. Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated With Tumor Growth Arrest

    • Timothy E. Baroni, Michele T. Lastro, Aparna C. Ranganathan, Scott A. Tenenbaum, Douglas S. Conklin, Julio A. Aguirre-Ghiso
    Pages 227-244

  17. Surface-Epitope Masking (SEM)

    • Neil I. Goldstein, Paul B. Fisher
    Pages 245-258

  18. Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells

    • Paul Dent, Philip B. Hylemon, Steven Grant, Paul B. Fisher
    Pages 259-276

  19. PKR in Innate Immunity, Cancer, and Viral Oncolysis

    • Siddharth Balachandran, Glen N. Barber
    Pages 277-301

  20. Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells

    • Hong Sun, Reshma Taneja
    Pages 303-310
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Keywords

About this book

Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects.

Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization.

Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.

Reviews

From the reviews:

"A compendium of techniques and applications that will be of wide utility to researchers interested in gene identification and function. … this volume will be of interest to a broad audience of molecular biologists, geneticists and biochemists and will provide a valuable resource in the laboratory due to its applicability to a wide breadth of projects. … for labs with a strong interest in proteomics and genomics it would certainly be well worth buying a copy to share. … I would certainly recommend this book." (Barbara Guinn, Immunology News, May, 2008)

Editors and Affiliations

  • Department of Pathology, Columbia University Medical Center, College of Physicians and Surgeons, New York

    Paul B. Fisher

  • Department of Neurosurgery, Columbia University Medical Center, College of Physicians and Surgeons, New York

    Paul B. Fisher

  • Department of Urology, Columbia University Medical Center, College of Physicians and Surgeons, New York

    Paul B. Fisher

Bibliographic Information

  • Book Title: Cancer Genomics and Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Paul B. Fisher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-335-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-504-0Published: 27 September 2007

  • Softcover ISBN: 978-1-61737-605-4Published: 19 November 2010

  • eBook ISBN: 978-1-59745-335-6Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 358

  • Number of Illustrations: 60 b/w illustrations

  • Topics: Oncology, Cancer Research, Human Genetics, Cell Biology

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