Skip to main content
SpringerLink
Log in
Book cover

Single Cell Diagnostics

Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Alan Thornhill

    1. The London Bridge Fertility, Gynecology and Genetics Centre, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Up-to-date, clinically applicable, validated protocols for single-cell diagnostics
  • Whole genome amplification to create starting material from single cells
  • Microarrays for analyzing multiple gene sequences simultaneously from a single cell
  • Non-invasive assessment via polymerase chain reaction and in situ hybridization

Part of the book series: Methods in Molecular Medicine (MIMM, volume 132)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Noninvasive Metabolic Assessment of Single Cells

    • David K. Gardner
    Pages 1-9

  3. Laser Capture Microdissection for Analysis of Single Cells

    • Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, Ricardo V. Lloyd
    Pages 11-18

  4. Fluorescence In Situ Hybridization on Single Cells

    • Paul N. Scriven, Caroline Mackie Ogilvie
    Pages 19-30

  5. Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis

    • Karen Sermon, Martine De Rycke
    Pages 31-42

  6. Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma

    • Bernhard Zimmermann, Xiao Yan Zhong, Wolfgang Holzgreve, Sinuhe Hahn
    Pages 43-49

  7. Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification

    • Jill L. Maron, Kirby L. Johnson, Diana W. Bianchi
    Pages 51-63

  8. Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies

    • Kenneth E. Pierce, Lawrence J. Wangh
    Pages 65-85

  9. Efficient Isothermal Amplification of the Entire Genome from Single Cells

    • Karen V. Schowalter, Jolene Fredrickson, Alan R. Thornhill
    Pages 87-99

  10. Comparative Genomic Hybridization on Single Cells

    • Lucille Voullaire, Leeanda Wilton
    Pages 101-115

  11. Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells

    • James Adjaye
    Pages 117-124

  12. Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells

    • Dagan Wells
    Pages 125-133

  13. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization

    • Dong Gui Hu, Xin Yuan Guan, Nicole Hussey
    Pages 135-151

  14. Microarray Technology for Mutation Analysis of Low-Template DNA Samples

    • Chelsea Salvado, David Cram
    Pages 153-173

  15. Back Matter

    Pages 175-182
    Download chapter PDF
Back to top

Keywords

About this book

The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

Reviews

From the reviews:

"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. … For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. … This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)

"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. … The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. … The chapters are written by experts and the book is carefully edited. … The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)

Editors and Affiliations

  • The London Bridge Fertility, Gynecology and Genetics Centre, London, UK

    Alan Thornhill

Bibliographic Information

  • Book Title: Single Cell Diagnostics

  • Book Subtitle: Methods and Protocols

  • Editors: Alan Thornhill

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1007/978-1-59745-298-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-578-1Published: 15 June 2007

  • Softcover ISBN: 978-1-61737-654-2Published: 10 November 2010

  • eBook ISBN: 978-1-59745-298-4Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XI, 182

  • Topics: Biochemistry, general, Human Genetics, Molecular Medicine, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation