Nuclear Transfer Protocols

1. Front Matter

   Pages i-xi

   PDF

2. Nuclear Transfer

   1. Front Matter

      Pages 197-197

      PDF

   2. Origin and Progress of Nuclear Transfer in Nonmammalian Animals

      • Marie A. Di Berardino

      Pages 3-31

   3. Culture and Maintenance of Nuclear Transfer Embryos

      1. In Vitro Maturation and Embryo Production in Cattle

         • Marc-André Sirard, Karine Coenen

         Pages 35-42

      2. Activation of Oocytes After Nuclear Transfer

         • Zoltán Macháty

         Pages 43-58

      3. Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos

         • Björn Heindryckx, Josiane Van der Elst, Marc Dhont

         Pages 59-77

      4. Maintenance of Pregnancy in Pigs With Limited Viable Embryos

         • Tim King, Paul A. De Sousa

         Pages 79-90

      5. Health and Neonatal Care of Bovine Clones

         • Jacqui T. Forsyth, David N. Wells

         Pages 91-108

   4. Nuclear Transfer Methods

      1. Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice

         • Young Gie Chung, Shaorong Gao, Keith E. Latham

         Pages 111-123

      2. Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors

         • Kazuchika Miyoshi, Sezen Arat, Steven L. Stice

         Pages 125-134

      3. A Double Nuclear Transfer Technique for Cloning Pigs

         • Irina A. Polejaeva, Shawn Walker, Keith Campbell

         Pages 135-149

      4. Nuclear Transfer in Nonhuman Primates

         • Shoukhrat M. Mitalipov, Don P. Wolf

         Pages 151-168

      5. Cloning of Exotic/Endangered Species: Desert Bighorn Sheep

         • James “Buck” Williams, Taeyoung Shin, Ling Liu, Gabriela Flores-Foxworth, Juan Romano, Alice Blue-McClendon et al.

         Pages 169-181

      6. Handmade Somatic Cell Cloning in Cattle

         • Gábor Vajta, Ian M. Lewis, R. Tayfur Tecirlioglu

         Pages 183-195

   5. Trangenesis

      1. Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells

         • A. John Clark, Sarah Burl, Chris Denning

         Pages 199-212

      2. Transgenesis Using Nuclear Transfer in Goats

         • Athoula Lazaris, Rebecca Keyston, Costas N. Karatzas, Carol L. Keefer

         Pages 213-226

3. Reprogramming Nuclei

   1. Front Matter

      Pages 227-227

      PDF

   2. Nuclear Reprogramming

      • Takashi Tada

      Pages 229-236

   3. Expression of Imprinted Genes in Cloned Mice

      • Takashi Kohda, Fumitoshi Ishino, Atsuo Ogura

      Pages 237-246

   4. Nuclear Remodeling Assay in Xenopus Egg Extract

      • Koichi Gonda, Nobuaki Kikyo

      Pages 247-258

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

• Activation
• gene expression
• gene transfer
• genes
• reproducible techniques
• vertebrates

"...an essential comprehensive handbook for both research and commercials labs involved in nuclear transfer, and will be of interest to molecular and stem cell biologists and biotechnologists involved with reprogramming, nuclear transfer and transgensis."  -Biotech-online

• Centre for Reproduction and Development Monash Institute of Medical Research, Monash University, Melbourne, Australia

  Paul J. Verma

• Monash Immunology and Stem Cell Laboratories, Monash University, Melbourne, Australia

  Alan O. Trounson

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