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Immunochemical Protocols

  • Book
  • © 1998

Overview

Editors:
  1. John D. Pound

    1. The University of Birmingham, UK

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 80)

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Table of contents (47 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Production of Polyclonal Antisera

    • Jonathan A. Green, Margaret M. Manson
    Pages 1-4

  3. Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen

    • Monique Diano, André Le Bivic, Michel Hirn
    Pages 5-13

  4. Production and Characterization of Antibodies Against Synthetic Peptides

    • David C. Hancock, Gerard I. Evan
    Pages 15-22

  5. Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins

    • Christopher J. Dean
    Pages 23-37

  6. A Simple Hollow-Fiber Bioreactor for the “In-House” Production of Monoclonal Antibodies

    • Margaret Goodall
    Pages 39-56

  7. Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase

    • Yveline Frobert, Jacques Grassi
    Pages 57-68

  8. Synthesis of Peptides for Use as Immunogens

    • David C. Hancock, Nicola J. O’ Reilly, Gerard I. Evan
    Pages 69-79

  9. Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens

    • Boguslaw Szewczyk, Donald F. Summers
    Pages 81-85

  10. Purification of Glycoproteins and Their Use as Immunogens

    • Boguslaw Szewczyk, Donald F. Summers
    Pages 87-93

  11. IgG Purification

    • Mark Page, Robin Thorpe
    Pages 95-111

  12. Purification of Monoclonal Antibodies

    • Mark Page, Robin Thorpe
    Pages 113-119

  13. Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives

    • Ruth R. French
    Pages 121-134

  14. Preparation of Cytotoxic Antibody—Toxin Conjugates

    • Alan J. Cumber, Edward J. Wawrzynczak
    Pages 135-144

  15. Immunoaffinity Purification and Quantification of Antibody—Toxin Conjugates

    • Edward J. Wawrzynczak, Alan J. Cumber
    Pages 145-153

  16. Competitive ELISA

    • Kittima Makarananda, Lucinda R. Weir, Gordon E. Neal
    Pages 155-160

  17. Epitope Mapping

    • Glenn E. Morris
    Pages 161-172

  18. Coupling of Antibodies with Biotin

    • Rosaria P. Haugland, Wendy W. You
    Pages 173-183

  19. Preparation of Avidin Conjugates

    • Rosaria P. Haugland, Mahesh K. Bhalgat
    Pages 185-196

  20. Enhanced Chemiluminescence Immunoassay

    • Richard A. W. Stott
    Pages 197-205
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About this book

This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates.

The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.

Reviews

"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."
-Microbiology Today

Editors and Affiliations

  • The University of Birmingham, UK

    John D. Pound

Bibliographic Information

  • Book Title: Immunochemical Protocols

  • Editors: John D. Pound

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59259-257-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • eBook ISBN: 978-1-59259-257-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 508

  • Topics: Cell Biology

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