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Chemically Defined Neural Conversion of Human Pluripotent Stem Cells

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Neural Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1919))

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Abstract

Human pluripotent stem cells (hPSCs) are characterized by their ability to self-renew and differentiate into any cell type of the human body. To fully utilize the potential of hPSCs for translational research and clinical applications, it is critical to develop rigorous cell differentiation protocols under feeder-free conditions that are efficient, reproducible, and scalable for high-throughput projects. Focusing on neural conversion of hPSCs, here we describe robust small molecule-based procedures that generate neural stem cells (NSCs) in less than a week under chemically defined conditions. These protocols can be used to dissect the mechanisms of neural lineage entry and to further develop systematic protocols that produce the cellular diversity of the central nervous system at industrial scale.

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Acknowledgment

We thank all our colleagues at the NIH National Center for Advancing Translational Sciences (NCATS) for their collaboration and the NIH Common Fund (Regenerative Medicine Program) for funding the Stem Cell Translation Laboratory (SCTL).

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Author notes

  1. Yu Chen and Carlos A. Tristan contributed equally to this work.

Authors and Affiliations

  1. Stem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA

    Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus, Anton Simeonov & Ilyas Singeç

Authors
  1. Yu Chen
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  2. Carlos A. Tristan
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  3. Sunil K. Mallanna
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  4. Pinar Ormanoglu
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  5. Steven Titus
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  6. Anton Simeonov
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  7. Ilyas Singeç
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Corresponding author

Correspondence to Ilyas Singeç .

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Editors and Affiliations

  1. Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA

    Marcel M. Daadi

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Chen, Y. et al. (2019). Chemically Defined Neural Conversion of Human Pluripotent Stem Cells. In: Daadi, M. (eds) Neural Stem Cells. Methods in Molecular Biology, vol 1919. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9007-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9007-8_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9005-4

  • Online ISBN: 978-1-4939-9007-8

  • eBook Packages: Springer Protocols

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