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Cellular Heterogeneity

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Natasha S. Barteneva

    1. PCMM-Harvard Medical School, Boston Children’s Hospital, Boston, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Ivan A. Vorobjev

    1. Department of Biology, School of Sciences and Technology, Nazarbayev University, Astana, Kazakhstan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of cellular heterogeneity
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1745)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xvi
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  2. General

    1. Front Matter

      Pages 1-1
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    2. Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level

      • Natasha S. Barteneva, Ivan A. Vorobjev
      Pages 3-23

    3. Integrating Analysis of Cellular Heterogeneity in High-Content Dose-Response Studies

      • Albert Gough, Tong Ying Shun, D. Lansing Taylor, Mark Schurdak
      Pages 25-46

    4. Image-Based Tracking of Heterogeneous Single-Cell Phenotypes

      • Katherin Patsch, Shannon M. Mumenthaler, Daniel Ruderman
      Pages 47-63

  3. Cytometry in Cellular Heterogeneity Research

    1. Front Matter

      Pages 65-65
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    2. Broad Immune Monitoring and Profiling of T Cell Subsets with Mass Cytometry

      • Tess Melinda Brodie, Vinko Tosevski
      Pages 67-82

    3. Spectral and Imaging Flow Cytometry in Phytoplankton Research

      • Veronika Dashkova, Jeff Clapper, Ivan A. Vorobjev, Natasha S. Barteneva
      Pages 83-95

    4. X-Ray Fluorescence-Detected Flow Cytometry

      • Andrew M. Crawford, James E. Penner-Hahn
      Pages 97-112

    5. Multiparametric Analysis of Myeloid Populations by Flow Cytometry

      • Sara A. Mathie, Alastair L. Corbin, Hayley L. Eames, Irina A. Udalova
      Pages 113-124

    6. Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry

      • Radiana T. Trifonova, Natasha S. Barteneva
      Pages 125-153

    7. Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry

      • Ekaterina Kopeikina, Marina Dukhinova, Eugene D. Ponomarev
      Pages 155-166

    8. Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System During Neuroinflammation and Neurodegeneration

      • Marina Dukhinova, Ekaterina Kopeikina, Eugene D. Ponomarev
      Pages 167-177

  4. Imaging and Spectroscopy Methods in Heterogeneity Studies

    1. Front Matter

      Pages 179-179
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    2. Analysis of Microtubule Dynamics Heterogeneity in Cell Culture

      • Anara Serikbaeva, Anna Tvorogova, Sholpan Kauanova, Ivan A. Vorobjev
      Pages 181-204

    3. Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts

      • Aleena Gladkikh, Anastasia Kovaleva, Anna Tvorogova, Ivan A. Vorobjev
      Pages 205-218

    4. Laser Tweezers Raman Microspectroscopy of Single Cells and Biological Particles

      • Maria Navas-Moreno, James W. Chan
      Pages 219-257

  5. Metabolic and Molecular Biological Methods in Cell Heterogeneity Tracking

    1. Front Matter

      Pages 259-259
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    2. Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically Encoded Sensor

      • Shabir Ahmad Bhat, Iram Khan Iqbal, Ashwani Kumar
      Pages 261-275

    3. Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites

      • Víctor Seco-Hidalgo, Antonio Osuna, Luis Miguel de Pablos
      Pages 277-286
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Keywords

About this book

This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.

Editors and Affiliations

  • PCMM-Harvard Medical School, Boston Children’s Hospital, Boston, USA

    Natasha S. Barteneva

  • Department of Biology, School of Sciences and Technology, Nazarbayev University, Astana, Kazakhstan

    Ivan A. Vorobjev

Bibliographic Information

  • Book Title: Cellular Heterogeneity

  • Book Subtitle: Methods and Protocols

  • Editors: Natasha S. Barteneva, Ivan A. Vorobjev

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7680-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7679-9Published: 24 February 2018

  • Softcover ISBN: 978-1-4939-8541-8Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7680-5Published: 23 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 363

  • Number of Illustrations: 14 b/w illustrations, 73 illustrations in colour

  • Topics: Cell Biology

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