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Schwann Cells

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Paula V. Monje

    1. Department of Neurological Surgery, University of Miami, Miami, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Haesun A. Kim

    1. Department of Biological Sciences, Rutgers University, Newark, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Include cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1739)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Protocols for Preparing Primary Cultures, Cell Lines and Engineered Schwann Cells

    1. Front Matter

      Pages 1-1
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    2. Isolation of Schwann Cell Precursors from Rodents

      • Rhona Mirsky, Kristjan R. Jessen
      Pages 3-15

    3. Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

      • Patrice Maurel
      Pages 17-37

    4. Isolation and Expansion of Schwann Cells from Transgenic Mouse Models

      • Jihyun Kim, Haesun A. Kim
      Pages 39-48

    5. Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers

      • Natalia D. Andersen, Paula V. Monje
      Pages 49-66

    6. Detailed Protocols for the Isolation, Culture, Enrichment and Immunostaining of Primary Human Schwann Cells

      • Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, Inge M. Ambros
      Pages 67-86

    7. Magnetic-Activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations

      • Kristine M. Ravelo, Natalia D. Andersen, Paula V. Monje
      Pages 87-109

    8. Culture and Expansion of Rodent and Porcine Schwann Cells for Preclinical Animal Studies

      • Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, Aisha Khan
      Pages 111-126

    9. Chemical Conversion of Human Fibroblasts into Functional Schwann Cells

      • Eva C. Thoma
      Pages 127-136

    10. Derivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats

      • Y. P. Tsui, Graham K. Shea, Y. S. Chan, Daisy K. Y. Shum
      Pages 137-148

    11. Human Induced Pluripotent Stem Cell-Derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-Derived Schwann Cells

      • Sa Cai, Daisy K. Y. Shum, Ying-Shing Chan
      Pages 149-160

    12. Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay

      • Alejandra M. Petrilli, Cristina Fernández-Valle
      Pages 161-173

  3. Protocols for Studying Schwann Cell Behavior and Myelination In Vitro

    1. Front Matter

      Pages 175-175
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    2. Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

      • Corey Heffernan, Patrice Maurel
      Pages 177-193

    3. Preservation, Sectioning, and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis

      • Vania W. Almeida, Margaret L. Bates, Mary Bartlett Bunge
      Pages 195-212

    4. Scalable Differentiation and Dedifferentiation Assays Using Neuron-Free Schwann Cell Cultures

      • Paula V. Monje
      Pages 213-232

    5. The Pseudopod System for Axon-Glia Interactions: Stimulation and Isolation of Schwann Cell Protrusions that Form in Response to Axonal Membranes

      • Yannick Poitelon, M. Laura Feltri
      Pages 233-253

    6. In Vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration Using Sensory Neurons from Dorsal Root Ganglia

      • Rodrigo López-Leal, Paula Diaz, Felipe A. Court
      Pages 255-267

    7. A Culture Model to Study Neuron-Schwann Cell-Astrocyte Interactions

      • Susana R. Cerqueira, Yee-Shuan Lee, Mary Bartlett Bunge
      Pages 269-279
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Keywords

About this book

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects.

Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells.

Editors and Affiliations

  • Department of Neurological Surgery, University of Miami, Miami, USA

    Paula V. Monje

  • Department of Biological Sciences, Rutgers University, Newark, USA

    Haesun A. Kim

Bibliographic Information

  • Book Title: Schwann Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Paula V. Monje, Haesun A. Kim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7649-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7648-5Published: 16 March 2018

  • Softcover ISBN: 978-1-4939-9254-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7649-2Published: 15 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 488

  • Number of Illustrations: 27 b/w illustrations, 68 illustrations in colour

  • Topics: Cell Biology

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