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Protein NMR

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Ranajeet Ghose

    1. Department of Chemistry and Biochemistry, The City College of New York, New York, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1688)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology

    • Caitlin M. Quinn, Mingzhang Wang, Tatyana Polenova
    Pages 1-35

  3. Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins

    • T. Gopinath, Gianluigi Veglia
    Pages 37-53

  4. Afterglow Solid-State NMR Spectroscopy

    • Gili Abramov, Nathaniel J. Traaseth
    Pages 55-66

  5. Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination

    • Omry Morag, Nikolaos G. Sgourakis, Gili Abramov, Amir Goldbourt
    Pages 67-97

  6. Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes

    • Erin R. Tyndall, Fang Tian
    Pages 99-109

  7. Rapid Prediction of Multi-dimensional NMR Data Sets Using FANDAS

    • Siddarth Narasimhan, Deni Mance, Cecilia Pinto, Markus Weingarth, Alexandre M. J. J. Bonvin, Marc Baldus
    Pages 111-132

  8. Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules

    • Boris Itin, Ivan V. Sergeyev
    Pages 133-154

  9. In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules

    • Yaewon Kim, Yunyi Wang, Hsueh-Ying Chen, Christian Hilty
    Pages 155-168

  10. Determination of Protein ps-ns Motions by High-Resolution Relaxometry

    • Samuel F. Cousin, Pavel Kadeřávek, Nicolas Bolik-Coulon, Fabien Ferrage
    Pages 169-203

  11. Characterizing Protein Dynamics with NMR R 1ρ Relaxation Experiments

    • Francesca Massi, Jeffrey W. Peng
    Pages 205-221

  12. CPMG Experiments for Protein Minor Conformer Structure Determination

    • Anusha B. Gopalan, D. Flemming Hansen, Pramodh Vallurupalli
    Pages 223-242

  13. Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR

    • Vincenzo Venditti, Nicolas L. Fawzi
    Pages 243-255

  14. From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis

    • Bruce A. Johnson
    Pages 257-310

  15. Protein Structure Elucidation from NMR Data with the Program Xplor-NIH

    • Guillermo A. Bermejo, Charles D. Schwieters
    Pages 311-340

  16. Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR

    • Mark W. Maciejewski, Adam D. Schuyler, Jeffrey C. Hoch
    Pages 341-352

  17. Covariance NMR Processing and Analysis for Protein Assignment

    • Bradley J. Harden, Dominique P. Frueh
    Pages 353-373

  18. Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System

    • Wolfgang Peti, Rebecca Page, Evzen Boura, Bartosz Różycki
    Pages 375-389

  19. Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist’s Approach to Allostery

    • Stephen Boulton, Rajeevan Selvaratnam, Rashik Ahmed, Giuseppe Melacini
    Pages 391-405

  20. High-Efficiency Expression of Yeast-Derived G-Protein Coupled Receptors and 19F Labeling for Dynamical Studies

    • Libin Ye, Alexander P. Orazietti, Aditya Pandey, R. Scott Prosser
    Pages 407-421
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Keywords

About this book

This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest.  Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise.

Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.

Reviews

“The book covers current applications of solid-state and solution nuclear magnetic resonance spectroscopy to study protein structure, dynamics. and interactions. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols with tips to tackle troubleshooting. The book has several high quality illustrations in color or black and white. … The intended audience appears to be students, specialists, and those who are interested in NMR spectroscopy.” (Omer Iqbal, Doody's Book Reviews, April, 2018)

Editors and Affiliations

  • Department of Chemistry and Biochemistry, The City College of New York, New York, USA

    Ranajeet Ghose

Bibliographic Information

  • Book Title: Protein NMR

  • Book Subtitle: Methods and Protocols

  • Editors: Ranajeet Ghose

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7386-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7385-9Published: 19 November 2017

  • Softcover ISBN: 978-1-4939-8469-5Published: 23 May 2018

  • eBook ISBN: 978-1-4939-7386-6Published: 18 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 446

  • Number of Illustrations: 62 illustrations in colour

  • Topics: Imaging / Radiology

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