Skip to main content
SpringerLink
Log in
Book cover

Duchenne Muscular Dystrophy

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Camilla Bernardini

    1. Istituto di Anatomia Umana e Biologia Cellulare, Università Cattolica del Sacro Cuore, Roma, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for DMD research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1687)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  3. Skeletal and Cardiac Muscle in DMD

    1. Front Matter

      Pages 29-29
      Download chapter PDF

    2. Cardiac Involvement in Duchenne Muscular Dystrophy and Related Dystrophinopathies

      • Sophie I. Mavrogeni, George Markousis-Mavrogenis, Antigoni Papavasiliou, George Papadopoulos, Genovefa Kolovou
      Pages 31-42

    3. Characterization of the Inflammatory Response in Dystrophic Muscle Using Flow Cytometry

      • Jenna M. Kastenschmidt, Ileen Avetyan, S. A. Villalta
      Pages 43-56

    4. Imaging Analysis of the Neuromuscular Junction in Dystrophic Muscle

      • Stephen J. P. Pratt, Shama R. Iyer, Sameer B. Shah, Richard M. Lovering
      Pages 57-72

  4. Omics Approaches

    1. Front Matter

      Pages 73-73
      Download chapter PDF

    2. System Biology Approach: Gene Network Analysis for Muscular Dystrophy

      • Federica Censi, Giovanni Calcagnini, Eugenio Mattei, Alessandro Giuliani
      Pages 75-89

    3. Proteomic Profiling of the Dystrophin-Deficient Brain

      • Sandra Murphy, Kay Ohlendieck
      Pages 91-105

    4. Probing the Pathogenesis of Duchenne Muscular Dystrophy Using Mouse Models

      • Alexander Morrison-Nozik, Saptarsi M. Haldar
      Pages 107-119

  5. Exon Skipping and Antisense Oligonucleotides (ASOs)

    1. Front Matter

      Pages 121-121
      Download chapter PDF

    2. Exon Skipping Therapy Using Phosphorodiamidate Morpholino Oligomers in the mdx52 Mouse Model of Duchenne Muscular Dystrophy

      • Shouta Miyatake, Yoshitaka Mizobe, Hotake Takizawa, Yuko Hara, Toshifumi Yokota, Shin’ichi Takeda et al.
      Pages 123-141

    3. Designing Effective Antisense Oligonucleotides for Exon Skipping

      • Takenori Shimo, Rika Maruyama, Toshifumi Yokota
      Pages 143-155

    4. Identification of Splicing Factors Involved in DMD Exon Skipping Events Using an In Vitro RNA Binding Assay

      • Julie Miro, Cyril F. Bourgeois, Mireille Claustres, Michel Koenig, Sylvie Tuffery-Giraud
      Pages 157-169

    5. The Use of Antisense Oligonucleotides for the Treatment of Duchenne Muscular Dystrophy

      • Karima Relizani, Aurelie Goyenvalle
      Pages 171-183

    6. PMO Delivery System Using Bubble Liposomes and Ultrasound Exposure for Duchenne Muscular Dystrophy Treatment

      • Yoichi Negishi, Yuko Ishii, Kei Nirasawa, Eri Sasaki, Yoko Endo-Takahashi, Ryo Suzuki et al.
      Pages 185-192
Back to top

Keywords

About this book

This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries achieved through in vivo gene editing which have proven to be promising in restoring dystrophin expression, at least in ameliorating skeletal muscle symptoms, and the contents focus on “Omics” techniques in gene expression, protein expression, miRNAs, and long non-coding RNA analysis, as well as experimental studies of the structural/functional changes affecting the skeletal and cardiac muscles and ongoing preclinical studies and clinical trials. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls. 

Authoritative and practical, Duchenne Muscular Dystrophy: Methods and Protocols serves as a guide for researchers exploring the complicated nature of dystrophin in the hope of helping the victims of this disorder.

Editors and Affiliations

  • Istituto di Anatomia Umana e Biologia Cellulare, Università Cattolica del Sacro Cuore, Roma, Italy

    Camilla Bernardini

Bibliographic Information

  • Book Title: Duchenne Muscular Dystrophy

  • Book Subtitle: Methods and Protocols

  • Editors: Camilla Bernardini

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7374-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7373-6Published: 25 October 2017

  • Softcover ISBN: 978-1-4939-8466-4Published: 23 August 2018

  • eBook ISBN: 978-1-4939-7374-3Published: 24 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 287

  • Number of Illustrations: 15 b/w illustrations, 34 illustrations in colour

  • Topics: Molecular Medicine

Publish with us

Policies and ethics

Back to top

Search

Navigation