Skip to main content
  • Book
  • © 2018

Synthetic Metabolic Pathways

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1671)

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Parts and Models for Designing Synthetic Metabolic Pathways

    1. Front Matter

      Pages 1-1
    2. Parts Characterization for Tunable Protein Expression

      • Michael S. Klausen, Morten O. A. Sommer
      Pages 3-14
    3. Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design

      • Jackson K. B. Cahn, Sabine Brinkmann-Chen, Frances H. Arnold
      Pages 15-26
    4. Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability

      • Ida Lauritsen, Virginia Martínez, Carlotta Ronda, Alex Toftgaard Nielsen, Morten H. H. Nørholm
      Pages 27-37
    5. Extended Metabolic Space Modeling

      • Pablo Carbonell, Baudoin Delépine, Jean-Loup Faulon
      Pages 83-96
    6. Computational Methods to Assess the Production Potential of Bio-Based Chemicals

      • Miguel A. Campodonico, Sumesh Sukumara, Adam M. Feist, Markus J. Herrgård
      Pages 97-116
  3. Building and Refactoring Synthetic Metabolic Pathways

    1. Front Matter

      Pages 117-117
    2. Multiplex Genome Editing in Escherichia coli

      • Sheila Ingemann Jensen, Alex Toftgaard Nielsen
      Pages 119-129
    3. CRISPR-Cas9 Toolkit for Actinomycete Genome Editing

      • Yaojun Tong, Helene Lunde Robertsen, Kai Blin, Tilmann Weber, Sang Yup Lee
      Pages 163-184
    4. Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR

      • Tadas Jakočiūnas, Emil D. Jensen, Michael K. Jensen, Jay D. Keasling
      Pages 185-201
  4. Screening and Selection of Synthetic Metabolic Pathways

    1. Front Matter

      Pages 227-227
    2. Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites for Phenotyping of Microorganisms

      • Christoph Halbfeld, Jörg Ingo Baumbach, Lars M. Blank, Birgitta E. Ebert
      Pages 229-258

About this book

This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Synthetic Metabolic Pathways: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • The Novo Nordisk Foundation, Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark

    Michael Krogh Jensen

  • Joint BioEnergy Institute, Emeryville, USA

    Jay D. Keasling

Bibliographic Information

  • Book Title: Synthetic Metabolic Pathways

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Krogh Jensen, Jay D. Keasling

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7295-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7294-4Published: 24 November 2017

  • Softcover ISBN: 978-1-4939-8445-9Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7295-1Published: 23 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 354

  • Number of Illustrations: 4 b/w illustrations, 64 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access