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Difference Gel Electrophoresis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Kay Ohlendieck

    1. Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1664)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Two-Dimensional Gel Electrophoresis and 2D-DIGE

      • Paula Meleady
      Pages 3-14

  3. Difference Gel Electrophoresis

    1. Front Matter

      Pages 15-15
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    2. Comparative DIGE Proteomics

      • Kay Ohlendieck
      Pages 17-24

    3. 2D-DIGE and Fluorescence Image Analysis

      • Elisa Robotti, Emilio Marengo
      Pages 25-39

    4. DIGE Analysis Software and Protein Identification Approaches

      • Abduladim Hmmier, Paul Dowling
      Pages 41-50

  4. DIGE Applications

    1. Front Matter

      Pages 51-51
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    2. Native DIGE: Efficient Tool to Elucidate Protein Interactomes

      • Diksha Dani, Norbert A. Dencher
      Pages 53-68

    3. Comparative Two-Dimensional Fluorescence Gel Electrophoresis

      • Doreen Ackermann, Simone König
      Pages 69-78

    4. DIGE-Based Phosphoproteomic Analysis

      • Taras Stasyk, Lukas Alfons Huber
      Pages 79-86

    5. DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue

      • Paul Dowling
      Pages 87-91

    6. Comparative 3-Sample DIGE Analysis of Skeletal Muscles

      • Kay Ohlendieck
      Pages 93-108

    7. DIGE Analysis of ProteoMinerTM Fractionated Serum/Plasma Samples

      • Sandra Murphy, Paul Dowling
      Pages 109-114

  5. Comparative DIGE Tissue Proteomics

    1. Front Matter

      Pages 115-115
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    2. DIGE Analysis of Human Tissues

      • Cecilia Gelfi, Daniele Capitanio
      Pages 117-136

    3. DIGE Analysis of Animal Tissues

      • Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, Giuliano Elia
      Pages 137-152

    4. Rapid 2D DIGE Proteomic Analysis of Mouse Liver

      • Shotaro Kamata, Isao Ishii
      Pages 153-166

    5. Proteomic Analysis of Lung Tissue by DIGE

      • Jarlath E. Nally, Simone Schuller
      Pages 167-183

    6. Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis

      • Ashling Holland
      Pages 185-202
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Keywords

About this book

This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland

    Kay Ohlendieck

Bibliographic Information

  • Book Title: Difference Gel Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Kay Ohlendieck

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7268-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7267-8Published: 11 October 2017

  • Softcover ISBN: 978-1-4939-8436-7Published: 22 August 2018

  • eBook ISBN: 978-1-4939-7268-5Published: 10 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 318

  • Number of Illustrations: 20 b/w illustrations, 24 illustrations in colour

  • Topics: Protein Science

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