Extracellular Vesicles

1. Front Matter

   Pages i-xvi

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2. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine

   • Mingyi Shang, John S. Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo et al.

   Pages 1-14

3. Red Blood Cells: A Source of Extracellular Vesicles

   • Winston Patrick Kuo, John C. Tigges, Vasilis Toxavidis, Ionita Ghiran

   Pages 15-22

4. Isolation of Extracellular Vesicles

   1. Front Matter

      Pages 23-23

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   2. Isolation of Extracellular Vesicles by Ultracentrifugation

      • Fatemeh Momen-Heravi

      Pages 25-32

   3. Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles

      • Mitja L. Heinemann, Jody Vykoukal

      Pages 33-41

   4. Paper-Based for Isolation of Extracellular Vesicles

      • Yi-Hsing Hsiao, Chihchen Chen

      Pages 43-54

   5. Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis

      • Cindy M. Yamamoto, Taku Murakami, Shu-Wing Ng

      Pages 55-63

   6. Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads

      • Ketil W. Pedersen, Bente Kierulf, Axl Neurauter

      Pages 65-87

5. Purification of Extracellular Vesicles

   1. Front Matter

      Pages 89-89

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   2. Polymer-Based Purification of Extracellular Vesicles

      • Peter N. Brown, Hang Yin

      Pages 91-103

   3. Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification

      • Richard Lobb, Andreas Möller

      Pages 105-110

   4. Purification Protocols for Extracellular Vesicles

      • Rebecca E. Lane, Darren Korbie, Matt Trau, Michelle M. Hill

      Pages 111-130

6. Characterization of Extracellular Vesicles

   1. Front Matter

      Pages 131-131

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   2. Characterization of Extracellular Vesicles by Surface Plasmon Resonance

      • Hyungsoon Im, Katherine Yang, Hakho Lee, Cesar M. Castro

      Pages 133-141

   3. Extracellular Vesicle Isolation and Analysis by Western Blotting

      • Emma J. K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, George M. Church

      Pages 143-152

   4. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis

      • Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, Rebecca Dragovic

      Pages 153-173

   5. Characterization of Extracellular Vesicles by Flow Cytometry

      • Virginia Camacho, Vasilis Toxavidis, John C. Tigges

      Pages 175-190

   6. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)

      • Tao Huang, Jiang He

      Pages 191-199

   7. Multi-Surface Antigen Staining of Larger Extracellular Vesicles

      • Veronika Lukacs-Kornek, Henrike Julich-Haertel, Sabine Katharina Urban, Miroslaw Kornek

      Pages 201-208

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.

• Biogenesis
• Exosomes
• Microvesicles
• Inter-cellular communication
• EVs
• Isolation
• Precision medicine

• CloudHealth Genomics, Ltd., Shanghai, China

  Winston Patrick Kuo

• Predicine, Inc., Hayward, USA

  Shidong Jia

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