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  • © 2017

mRNA Processing

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for the study of mRNA processing
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1648)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA

    • Eimile Oakes, Pranathi Vadlamani, Heather A. Hundley
    Pages 103-127
  3. Using RNA-Seq to Discover Genetic Polymorphisms That Produce Hidden Splice Variants

    • Shayna Stein, Emad Bahrami-Samani, Yi Xing
    Pages 129-142
  4. Inducible Expression of Eukaryotic Circular RNAs from Plasmids

    • Deirdre C. Tatomer, Dongming Liang, Jeremy E. Wilusz
    Pages 143-154
  5. Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine

    • Angela Garibaldi, Francisco Carranza, Klemens J. Hertel
    Pages 169-176
  6. Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)

    • Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt et al.
    Pages 177-200Open Access
  7. Assaying RNA Structure Inside Living Cells with SHAPE

    • Chao Feng, Dalen Chan, Robert C. Spitale
    Pages 247-256
  8. Erratum to: Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)

    • Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt et al.
    Pages E1-E1

About this book

This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what’s certain to be another explosive period of growth in this dynamic field of study.


The chapter 'Robust, Cost-EffectiveProfiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)' is published open access under a CC BY 4.0 license at link.springer.com. 

Editors and Affiliations

  • Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, USA

    Yongsheng Shi

Bibliographic Information

  • Book Title: mRNA Processing

  • Book Subtitle: Methods and Protocols

  • Editors: Yongsheng Shi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7204-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7203-6Published: 02 August 2017

  • Softcover ISBN: 978-1-4939-8415-2Published: 12 August 2018

  • eBook ISBN: 978-1-4939-7204-3Published: 01 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 258

  • Number of Illustrations: 16 b/w illustrations, 33 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access