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  • © 2017

RNAi and Small Regulatory RNAs in Stem Cells

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for the study of the intersection of RNAi, small regulatory RNAs, and stem cell biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1622)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Introduction

    1. Front Matter

      Pages 1-1
  3. RNA Interference

    1. Front Matter

      Pages 27-27
    2. Forward RNAi Screens in Human Hematopoietic Stem Cells

      • Roman Galeev, Christine Karlsson, Aurélie Baudet, Jonas Larsson
      Pages 29-50
    3. Silencing of ATP11B by RNAi-Induced Changes in Neural Stem Cell Morphology

      • Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, Tieqiao Wen
      Pages 51-61
    4. High-Efficiency Transfection of Glioblastoma Cells and a Simple Spheroid Migration Assay

      • Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, Mario Löhr
      Pages 63-79
    5. Efficient Depletion of Essential Gene Products for Loss-of-Function Studies in Embryonic Stem Cells

      • Soizik Berlivet, Isabelle Hmitou, Hélène Picaud, Matthieu Gérard
      Pages 91-100
    6. RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells

      • Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, Yumei Fu
      Pages 131-138
    7. Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry

      • Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola et al.
      Pages 149-159
    8. RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells

      • Amelia J. Blake, Danielle S. Finger, Victoria L. Hardy, Elizabeth T. Ables
      Pages 161-184
  4. MicroRNAs

    1. Front Matter

      Pages 223-223

About this book

This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature.

Editors and Affiliations

  • Department of Biology, East Carolina University, Greenville, USA

    Baohong Zhang

Bibliographic Information

  • Book Title: RNAi and Small Regulatory RNAs in Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Baohong Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7108-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-7106-0Published: 03 July 2017

  • Softcover ISBN: 978-1-4939-8386-5Published: 12 June 2018

  • eBook ISBN: 978-1-4939-7108-4Published: 03 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 311

  • Number of Illustrations: 18 b/w illustrations, 32 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access