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T-Cell Trafficking

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. George Edward Rainger

    1. Institute of Cardiovascular Sciences, University of Birmingham, Birmingham, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Helen M. Mcgettrick

    1. Institute of Inflammation and Ageing, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1591)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Homeostatic Migration

    • Mark C. Coles
    Pages 1-8

  3. Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multiphoton Fluorescence Microscopy of Live Thymic Slices

    • Jessica N. Lancaster, Lauren I. R. Ehrlich
    Pages 9-25

  4. Visualizing and Tracking T Cell Motility In Vivo

    • Robert A. Benson, James M. Brewer, Paul Garside
    Pages 27-41

  5. Graph Theory-Based Analysis of the Lymph Node Fibroblastic Reticular Cell Network

    • Mario Novkovic, Lucas Onder, Gennady Bocharov, Burkhard Ludewig
    Pages 43-57

  6. Visualizing Endogenous Effector T Cell Egress from the Lymph Nodes

    • Manisha Menon, Alexandre P. Benechet, Kamal M. Khanna
    Pages 59-71

  7. Introduction: T Cell Trafficking in Inflammation and Immunity

    • Myriam Chimen, Bonita H. R. Apta, Helen M. Mcgettrick
    Pages 73-84

  8. Leukocyte Adhesion Under Hemodynamic Flow Conditions

    • Charlotte Lawson, Marlene Rose, Sabine Wolf
    Pages 85-100

  9. Endocrine Regulation of Lymphocyte Trafficking In Vitro

    • Bonita H. R. Apta, Myriam Chimen, Helen M. Mcgettrick
    Pages 101-119

  10. Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells

    • Helen M. Mcgettrick, Lewis S. C. Ward, George Edward Rainger, Gerard B. Nash
    Pages 121-142

  11. Monitoring RhoGTPase Activity in Leukocytes Using Classic “Pull-Down” Assays

    • Marouan Zarrouk, David Killock, Izajur Rahman, Jessica Davies, Aleksandar Ivetić
    Pages 143-153

  12. Utilizing Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions

    • Jasmeet S. Reyat, Michael G. Tomlinson, Peter J. Noy
    Pages 155-168

  13. Introduction to Lymphocyte Trafficking in Disease

    • Patricia F. Lalor, Elizabeth A. Hepburn
    Pages 169-176

  14. Using Ex Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking

    • Benjamin G. Wiggins, Zania Stamataki, Patricia F. Lalor
    Pages 177-194

  15. In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

    • Benjamin G. Wiggins, Konstantinos Aliazis, Scott P. Davies, Gideon Hirschfield, Patricia F. Lalor, Gary Reynolds et al.
    Pages 195-214

  16. Monitoring Migration of Activated T Cells to Antigen-Rich Non-lymphoid Tissue

    • Eleanor Jayne Ward, Hongmei Fu, Federica Marelli-Berg
    Pages 215-224

  17. Tissue Digestion for Stromal Cell and Leukocyte Isolation

    • Saba Nayar, Joana Campos, Nathalie Steinthal, Francesca Barone
    Pages 225-234

  18. T Cell Response in the Lung Following Influenza Virus Infection

    • Robert A. Benson, Jennifer C. Lawton, Megan K. L. MacLeod
    Pages 235-248

  19. Back Matter

    Pages 249-252
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Keywords

About this book

This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during ‘physiological’ resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.

Editors and Affiliations

  • Institute of Cardiovascular Sciences, University of Birmingham, Birmingham, United Kingdom

    George Edward Rainger

  • Institute of Inflammation and Ageing, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

    Helen M. Mcgettrick

Bibliographic Information

  • Book Title: T-Cell Trafficking

  • Book Subtitle: Methods and Protocols

  • Editors: George Edward Rainger, Helen M. Mcgettrick

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6931-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6929-6Published: 28 March 2017

  • Softcover ISBN: 978-1-4939-8342-1Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6931-9Published: 27 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 252

  • Number of Illustrations: 15 b/w illustrations, 32 illustrations in colour

  • Topics: Immunology

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