Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Protein-Carbohydrate Interactions

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. D. Wade Abbott

    1. Agriculture and Agri-Food Canada, Lethbridge, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Alicia Lammerts van Bueren

    1. Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1588)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Analysis of Carbohydrate-Active Enzyme Activity

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases

      • Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, Harry Brumer
      Pages 3-14

    3. Quantitative Kinetic Characterization of Glycoside Hydrolases Using High-Performance Anion-Exchange Chromatography (HPAEC)

      • Nicholas McGregor, Gregory Arnal, Harry Brumer
      Pages 15-25

    4. Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay

      • Lauren S. McKee
      Pages 27-36

    5. An Improved Kinetic Assay for the Characterization of Metal-Dependent Pectate Lyases

      • Darryl R. Jones, Richard McLean, D. Wade Abbott
      Pages 37-44

    6. Colorimetric Detection of Acetyl Xylan Esterase Activities

      • Galina Mai-Gisondi, Emma R. Master
      Pages 45-57

    7. Methods for Determining Glycosyltransferase Kinetics

      • Maria Ngo, Michael D. L. Suits
      Pages 59-70

    8. Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry

      • Bjørge Westereng, Magnus Ø. Arntzen, Jane Wittrup Agger, Gustav Vaaje-Kolstad, Vincent G. H. Eijsink
      Pages 71-92

    9. Carbohydrate Depolymerization by Intricate Cellulosomal Systems

      • Johanna Stern, Lior Artzi, Sarah Moraïs, Carlos M. G. A. Fontes, Edward A. Bayer
      Pages 93-116

  3. Analysis of Protein-Carbohydrate Interactions

    1. Front Matter

      Pages 117-117
      Download chapter PDF

    2. Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions

      • Darrell Cockburn, Casper Wilkens, Birte Svensson
      Pages 119-127

    3. Quantifying CBM Carbohydrate Interactions Using Microscale Thermophoresis

      • Haiyang Wu, Cédric Y. Montanier, Claire Dumon
      Pages 129-141

    4. Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy

      • Julie M. Grondin, David N. Langelaan, Steven P. Smith
      Pages 143-156

    5. Measuring the Biomechanical Loosening Action of Bacterial Expansins on Paper and Plant Cell Walls

      • Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, Daniel M. Durachko
      Pages 157-165

  4. Visualization of Carbohydrates and Protein-Carbohydrate Complexes

    1. Front Matter

      Pages 167-167
      Download chapter PDF

    2. Bioinspired Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP

      • Gabriel Paës
      Pages 169-179

    3. CBMs as Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry

      • Louise Badruna, Vincent Burlat, Cédric Y. Montanier
      Pages 181-197

    4. Determining the Localization of Carbohydrate Active Enzymes Within Gram-Negative Bacteria

      • Richard McLean, G. Douglas Inglis, Steven C. Mosimann, Richard R. E. Uwiera, D. Wade Abbott
      Pages 199-208

    5. Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR)

      • Ajay Badhan, Yuxi Wang, Tim A. McAllister
      Pages 209-214
Back to top

Keywords

About this book

This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes: Part I describes methods for screening and quantifying CAZyme activity; Part II contains methods for investigating the interactions between proteins and carbohydrate ligands; Part III discusses methods for the visualization of carbohydrates and protein-carbohydrate complexes; and Part IV focuses on structural and “omic” approaches for studying systems of CAZymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein-Carbohydrate Interactions: Methods and Protocols is a valuable resource to the glycomics research community. In this continuously advancing field, the methods in this book highlight the biology of glycomics, thus driving biotechnological innovation and solutions for human health and sustainable resources within the emerging green community.

Editors and Affiliations

  • Agriculture and Agri-Food Canada, Lethbridge, Canada

    D. Wade Abbott

  • Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    Alicia Lammerts van Bueren

Bibliographic Information

  • Book Title: Protein-Carbohydrate Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: D. Wade Abbott, Alicia Lammerts van Bueren

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6899-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6898-5Published: 18 April 2017

  • Softcover ISBN: 978-1-4939-8332-2Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6899-2Published: 17 April 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 311

  • Number of Illustrations: 20 b/w illustrations, 42 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation