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  • © 2017

Plant Pattern Recognition Receptors

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice form the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1578)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
  2. Characterization of Plant Cell Wall Damage-Associated Molecular Patterns Regulating Immune Responses

    • Laura Bacete, Hugo Mélida, Sivakumar Pattathil, Michael G. Hahn, Antonio Molina, Eva Miedes
    Pages 13-23
  3. Methods of Isolation and Characterization of Oligogalacturonide Elicitors

    • Manuel Benedetti, Benedetta Mattei, Daniela Pontiggia, Gianni Salvi, Daniel Valentin Savatin, Simone Ferrari
    Pages 25-38
  4. Assays to Investigate the N-Glycosylation State and Function of Plant Pattern Recognition Receptors

    • Stacey A. Lawrence, Teresa Ceserani, Nicole K. Clay
    Pages 61-79
  5. In Vitro Ubiquitination Activity Assays in Plant Immune Responses

    • Giulia Furlan, Marco Trujillo
    Pages 109-121
  6. Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily

    • Otávio J. B. Brustolini, José Cleydson F. Silva, Tetsu Sakamoto, Elizabeth P. B. Fontes
    Pages 123-132
  7. Identification of MAPK Substrates Using Quantitative Phosphoproteomics

    • Tong Zhang, Jacqueline D. Schneider, Ning Zhu, Sixue Chen
    Pages 133-142
  8. Analysis of PAMP-Triggered ROS Burst in Plant Immunity

    • Yuying Sang, Alberto P. Macho
    Pages 143-153
  9. MAPK Assays in Arabidopsis MAMP-PRR Signal Transduction

    • Hoo Sun Chung, Jen Sheen
    Pages 155-166
  10. LeEIX2 Interactors’ Analysis and EIX-Mediated Responses Measurement

    • Meirav Leibman-Markus, Silvia Schuster, Adi Avni
    Pages 167-172
  11. CDPK Activation in PRR Signaling

    • Heike Seybold, Marie Boudsocq, Tina Romeis
    Pages 173-183
  12. Chitin and Stress Induced Protein Kinase Activation

    • Chandra Kenchappa, Raquel Azevedo da Silva, Simon Bressendorff, Sabrina Stanimirovic, Jakob Olsen, Morten Petersen et al.
    Pages 185-194

About this book

This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Department of Plant Pathology and Microbiology Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, USA

    Libo Shan

  • Department of Biochemistry and Biophysics Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, USA

    Ping He

Bibliographic Information

  • Book Title: Plant Pattern Recognition Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Libo Shan, Ping He

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6859-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6858-9Published: 02 March 2017

  • Softcover ISBN: 978-1-4939-8320-9Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6859-6Published: 20 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 358

  • Number of Illustrations: 18 b/w illustrations, 39 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access