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  • © 2017

Muscle Stem Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1556)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Overview

    1. Front Matter

      Pages 1-1
    2. Muscle Stem Cells: A Model System for Adult Stem Cell Biology

      • DDW Cornelison, Eusebio Perdiguero
      Pages 3-19
  3. Muscle Stem Cells (Satellite cells)

    1. Front Matter

      Pages 21-21
    2. Isolation of Muscle Stem Cells from Mouse Skeletal Muscle

      • Barbara Gayraud-Morel, Francesca Pala, Hiroshi Sakai, Shahragim Tajbakhsh
      Pages 23-39
    3. Primary Mouse Myoblast Purification using Magnetic Cell Separation

      • Marie Claude Sincennes, Yu Xin Wang, Michael A. Rudnicki
      Pages 41-50
    4. Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors

      • Guillaume Lavergne, Cedric Soler, Monika Zmojdzian, Krzysztof Jagla
      Pages 103-116
    5. Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells

      • Phong D. Nguyen, Peter D. Currie
      Pages 117-125
  4. Other Muscle Interstitial Stem Cells

    1. Front Matter

      Pages 127-127
    2. Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle

      • Francesco Saverio Tedesco, Louise A. Moyle, Eusebio Perdiguero
      Pages 129-147
    3. Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle

      • Rossana Tonlorenzi, Giuliana Rossi, Graziella Messina
      Pages 149-177
    4. Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture

      • Marcela Low, Christine Eisner, Fabio Rossi
      Pages 179-189
    5. Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells

      • Sole Gatto, Pier Lorenzo Puri, Barbora Malecova
      Pages 191-219
  5. Assays for Stem Cell Functionality

    1. Front Matter

      Pages 221-221
    2. Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle

      • Matthew Tierney, Alessandra Sacco
      Pages 223-236
    3. Transplantation of Skeletal Muscle Stem Cells

      • Monica N. Hall, John K. Hall, Adam B. Cadwallader, Bradley T. Pawlikowski, Jason D. Doles, Tiffany L. Elston et al.
      Pages 237-244
    4. Monitoring Autophagy in Muscle Stem Cells

      • Laura García-Prat, Pura Muñoz-Cánoves, Marta Martínez-Vicente
      Pages 255-280
  6. Cell culture, In Vitro, and In Silico Studies

    1. Front Matter

      Pages 281-281

About this book

This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduction in vivo as well as protocols for studying myogenic stem cells in non-mammalian model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Muscle Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Cell Biology Group, Department of Experimental and Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain

    Eusebio Perdiguero

  • Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, USA

    DDW Cornelison

Bibliographic Information

  • Book Title: Muscle Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Eusebio Perdiguero, DDW Cornelison

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6771-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6769-8Published: 02 March 2017

  • Softcover ISBN: 978-1-4939-8296-7Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6771-1Published: 27 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 365

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access