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Multiplex Biomarker Techniques

Methods and Applications

  • Book
  • © 2017

Overview

Editors:
  1. Paul C. Guest

    1. Laboratory of Neuroproteomics, University of Campinas (UNICAMP), Campinas, Brazil

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  • Includes cutting-edge methods and protocols for the study of multiplex biomarker research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1546)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development

      • Hassan Rahmoune, Paul C. Guest
      Pages 3-17

    3. The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients

      • Johann Steiner, Paul C. Guest, Hassan Rahmoune, Daniel Martins-de-Souza
      Pages 19-35

    4. Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research

      • Susan E. Ozanne, Hassan Rahmoune, Paul C. Guest
      Pages 37-55

    5. LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics

      • Gustavo Henrique Martins Ferreira Souza, Paul C. Guest, Daniel Martins-de-Souza
      Pages 57-73

    6. Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies

      • James R. Mansfield
      Pages 75-99

  3. Statistical Considerations

    1. Front Matter

      Pages 101-101
      Download chapter PDF

    2. Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations

      • Emanuel Schwarz
      Pages 103-114

    3. Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective

      • Junfang Chen, Emanuel Schwarz
      Pages 115-122

  4. Protocols

    1. Front Matter

      Pages 123-123
      Download chapter PDF

    2. Multiplex Analyses Using Real-Time Quantitative PCR

      • Steve F. C. Hawkins, Paul C. Guest
      Pages 125-133

    3. Multiplex Analysis Using cDNA Transcriptomic Profiling

      • Steve F. C. Hawkins, Paul C. Guest
      Pages 135-142

    4. Multiplex Single Nucleotide Polymorphism Analyses

      • Steve F. C. Hawkins, Paul C. Guest
      Pages 143-148

    5. Pulsed SILAC as a Approach for miRNA Targets Identification in Cell Culture

      • Daniella E. Duque-Guimarães, Juliana de Almeida-Faria, Thomas Prates Ong, Susan E. Ozanne
      Pages 149-159

    6. Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies

      • Paul C. Guest, Hassan Rahmoune
      Pages 161-168

    7. Multiplex Immunoassay Profiling

      • Laurie Stephen
      Pages 169-176

    8. Multiplex Sequential Immunoprecipitation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets

      • Paul C. Guest
      Pages 177-185

    9. Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets

      • Paul C. Guest
      Pages 187-194

    10. Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders

      • Sheila Garcia, Paulo A. Baldasso, Paul C. Guest, Daniel Martins-de-Souza
      Pages 195-204
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Keywords

About this book

This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Multiplex Biomarker Techniques: Methods and Applications serves as an ideal resource for a wide variety of researchers interested in these vital multiplex techniques.

Editors and Affiliations

  • Laboratory of Neuroproteomics, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

Bibliographic Information

  • Book Title: Multiplex Biomarker Techniques

  • Book Subtitle: Methods and Applications

  • Editors: Paul C. Guest

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6730-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6729-2Published: 29 November 2016

  • Softcover ISBN: 978-1-4939-8285-1Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6730-8Published: 25 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 317

  • Number of Illustrations: 11 b/w illustrations, 48 illustrations in colour

  • Topics: Pharmacology/Toxicology

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