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Exosomes and Microvesicles

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Andrew F Hill

    1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1545)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Methods to Analyze EVs

    • Bernd Giebel, Clemens Helmbrecht
    Pages 1-20

  3. Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles

    • Sybren L. N. Maas, Marike L. D. Broekman, Jeroen de Vrij
    Pages 21-33

  4. Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis

    • Kym McNicholas, Michael Z. Michael
    Pages 35-42

  5. Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy

    • Romain Linares, Sisareuth Tan, Céline Gounou, Alain R. Brisson
    Pages 43-54

  6. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR

    • Shayne A. Bellingham, Mitch Shambrook, Andrew F. Hill
    Pages 55-70

  7. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ and Ion S5™ System

    • Lesley Cheng, Andrew F. Hill
    Pages 71-90

  8. A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration

    • Rong Xu, Richard J. Simpson, David W. Greening
    Pages 91-116

  9. Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array)

    • Rikke Bæk, Malene Møller Jørgensen
    Pages 117-127

  10. Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation

    • Karine Laulagnier, Charlotte Javalet, Fiona J. Hemming, Rémy Sadoul
    Pages 129-138

  11. A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space

    • Rocío Pérez-González, Sebastien A. Gauthier, Asok Kumar, Mitsuo Saito, Mariko Saito, Efrat Levy
    Pages 139-151

  12. Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission

    • Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann et al.
    Pages 153-176

  13. Isolation of Platelet-Derived Extracellular Vesicles

    • Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland, Pia Siljander
    Pages 177-188

  14. Bioinformatics Tools for Extracellular Vesicles Research

    • Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, Suresh Mathivanan
    Pages 189-196

  15. Preparation and Isolation of siRNA-Loaded Extracellular Vesicles

    • Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E. L. Andaloussi, Matthew J. A. Wood
    Pages 197-204

  16. Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions

    • Susan M. van Dommelen, Margaret Fish, Arjan D. Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, Pieter Vader
    Pages 205-213

  17. Flow Cytometric Analysis of Extracellular Vesicles

    • Aizea Morales-Kastresana, Jennifer C. Jones
    Pages 215-225

  18. Back Matter

    Pages 227-228
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Keywords

About this book

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.

 

Editors and Affiliations

  • Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia

    Andrew F Hill

About the editor

Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute, 
Email: a.hill@unimelb.edu.au

Bibliographic Information

  • Book Title: Exosomes and Microvesicles

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew F Hill

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6728-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6726-1Published: 10 December 2016

  • Softcover ISBN: 978-1-4939-8284-4Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6728-5Published: 09 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 228

  • Number of Illustrations: 18 b/w illustrations, 21 illustrations in colour

  • Topics: Cell Biology

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