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Small Molecule Microarrays

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1518)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. The Expanding World of Small Molecule Microarrays

    • Mahesh Uttamchandani, Shao Q. Yao
    Pages 1-17
  3. Novel Substrates for Microarrays

    • Dameng Guo, Han Wu, Liang Wu, Bo Zheng
    Pages 19-28
  4. Fabrication of Carbohydrate Microarrays by Boronate Formation

    • Avijit K. Adak, Ting-Wei Lin, Ben-Yuan Li, Chun-Cheng Lin
    Pages 43-53
  5. Clickable Polymeric Coating for Glycan Microarrays

    • Caterina Zilio, Laura Sola, Marina Cretich, Anna Bernardi, Marcella Chiari
    Pages 55-65
  6. A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene

    • Ping Wang, Liqian Gao, Haipeng Lei, Su Seong Lee, Shao Q. Yao, Hongyan Sun
    Pages 67-80
  7. Label-Free Sensing on Microarrays

    • Yung-Shin Sun
    Pages 81-108
  8. Optical Microscopy for Detecting Binding on Small Molecule Microarrays

    • Si Ying Png, Sherlyn Xiang Bing Seah, Zhiqiang Gao
    Pages 109-129
  9. Array-on-Array Strategy For Activity-Based Enzyme Profiling

    • Brendan Fu-Long Sieow, Mahesh Uttamchandani
    Pages 131-138
  10. Protein–Protein Interaction Inhibitors of BRCA1 Discovered Using Small Molecule Microarrays

    • Zhenkun Na, Sijun Pan, Mahesh Uttamchandani, Shao Q. Yao
    Pages 139-156
  11. Discovery of RNA Binding Small Molecules Using Small Molecule Microarrays

    • Colleen M. Connelly, Fardokht A. Abulwerdi, John S. Schneekloth Jr.
    Pages 157-175
  12. Profiling Phosphopeptide-Binding Domain Recognition Specificity Using Peptide Microarrays

    • Michele Tinti, Simona Panni, Gianni Cesareni
    Pages 177-193
  13. Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-Based Chemiluminescence Microarrays

    • Verena Katharina Meyer, Daniela Meloni, Fabio Olivo, Erwin Märtlbauer, Richard Dietrich, Reinhard Niessner et al.
    Pages 195-212
  14. Synthetic Glycan Microarrays

    • Felix Broecker, Peter H. Seeberger
    Pages 227-240
  15. Screening Mammalian Cells on a Hydrogel: Functionalized Small Molecule Microarray

    • Biwei Zhu, Bo Jiang, Zhenkun Na, Shao Q. Yao
    Pages 241-255
  16. Back Matter

    Pages 257-260

About this book

Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide researchers toward ways in which SMM technology may be effectively deployed for multiplexed screening, drug discovery and ligand identification. The volume is organized into three sections covering microarray fabrication, imaging modes, and discovery approaches. The chapters cover a range of small molecule library types, ranging from synthetic combinatorial libraries to peptide and carbohydrate libraries, with lessons and techniques that can be widely applied. Written in the highly successful Methods in Molecular Biology series, format, chapters include introductions of their respective topics, lists of the requisite materials and reagents, step-by-step and readily reproducible laboratory protocols as well as vital tips on troubleshooting and advice on avoiding known pitfalls.

 

Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous insights into the successful applications of SMM, by experts in the field. Building on this collection of knowledge, the volume seeks to inspire a future generation of microarray practitioners to propel this significant technology to even greater heights

 

Editors and Affiliations

  • DSO National Laboratories, Defence Medical and Environmental Resear, Singapore, Singapore

    Mahesh Uttamchandani

  • Department of Chemistry Faculty of Science, National University of Singapore, Singapore, Singapore

    Shao Q. Yao

Bibliographic Information

  • Book Title: Small Molecule Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Mahesh Uttamchandani, Shao Q. Yao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6584-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6582-3Published: 21 November 2016

  • Softcover ISBN: 978-1-4939-8242-4Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6584-7Published: 19 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 260

  • Number of Illustrations: 22 b/w illustrations, 60 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access