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The Mitotic Exit Network

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Fernando Monje-Casas

    1. CABIMER, Sevilla, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Ethel Queralt

    1. Cancer Epigenetics & Biology Program, IDIBELL Hospitalet de Llobregat, Barcelona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for studying the Mitotic Exit Network
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1505)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
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  2. Analysis of Cell Cycle Progression and Mitotic Exit

    1. Front Matter

      Pages 1-1
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    2. Regulation of Mitotic Exit in Saccharomyces cerevisiae

      • Bàrbara Baro, Ethel Queralt, Fernando Monje-Casas
      Pages 3-17

    3. Methods of Synchronization of Yeast Cells for the Analysis of Cell Cycle Progression

      • M. Angeles Juanes
      Pages 19-34

    4. Analysis of Cell Cycle Progression in Saccharomyces cerevisiae Using the Budding Index and Tubulin Staining

      • Marta Muñoz-Barrera, Fernando Monje-Casas
      Pages 35-44

    5. Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators

      • Cristina Cepeda-García
      Pages 45-57

    6. Cdc14 Localization as a Marker for Mitotic Exit: In Vivo Quantitative Analysis of Cdc14 Release

      • Gabriel Neurohr, Manuel Mendoza
      Pages 59-67

  3. Biochemistry of the MEN

    1. Front Matter

      Pages 69-69
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    2. In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP

      • Marco Geymonat, Adonis Spanos, Katrin Rittinger
      Pages 71-80

    3. Analysis of Protein–Protein Interactions Between MEN Components

      • Rami Rahal
      Pages 81-88

    4. A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity

      • Ethel Queralt, José-Antonio Rodriguez-Rodriguez
      Pages 89-96

    5. Analysis of SUMOylation in the RENT Complex by Fusion to a SUMO-Specific Protease Domain

      • Neus Colomina, Clàudia Guasch, Jordi Torres-Rosell
      Pages 97-117

    6. A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets

      • Brendan L. Powers, Hana Hall, Harry Charbonneau, Mark C. Hall
      Pages 119-132

  4. Analysis of the Localization of MEN Components

    1. Front Matter

      Pages 133-133
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    2. Localizing MEN Components by Indirect Immunofluorescence Analysis of Budding Yeast

      • Francesca Senic-Matuglia, Rosella Visintin
      Pages 135-149

    3. Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy

      • Zhiang Guo, Marisa Segal
      Pages 151-166

    4. Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP)

      • Ayse Koca Caydasi, Gislene Pereira
      Pages 167-182

    5. Asymmetric Localization of Components and Regulators of the Mitotic Exit Network at Spindle Pole Bodies

      • Ilaria Scarfone, Simonetta Piatti
      Pages 183-193

    6. Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays

      • Julie Huang, Nahid Iglesias, Danesh Moazed
      Pages 195-213

  5. Control of Cellular Processes by the MEN

    1. Front Matter

      Pages 215-215
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Keywords

About this book

This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods inMolecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.

Editors and Affiliations

  • CABIMER, Sevilla, Spain

    Fernando Monje-Casas

  • Cancer Epigenetics & Biology Program, IDIBELL Hospitalet de Llobregat, Barcelona, Spain

    Ethel Queralt

Bibliographic Information

  • Book Title: The Mitotic Exit Network

  • Book Subtitle: Methods and Protocols

  • Editors: Fernando Monje-Casas, Ethel Queralt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6502-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6500-7Published: 09 November 2016

  • Softcover ISBN: 978-1-4939-8220-2Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6502-1Published: 08 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 281

  • Number of Illustrations: 11 b/w illustrations, 24 illustrations in colour

  • Topics: Cell Biology

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