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Mammary Gland Development

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Finian Martin

    1. School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Torsten Stein

    1. Institute of Cancer Sciences College of MVLS University of Glasgow, Glasgow, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  3. Jillian Howlin

    1. Division of Oncology-Pathology, Lund University Cancer Center/Medicon Village, Lund, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1501)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Overview of Mammary Gland Development: A Comparison of Mouse and Human

    • Sara McNally, Torsten Stein
    Pages 1-17

  3. In Vivo Models

    1. Front Matter

      Pages 19-19
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    2. Prenatal Mammary Gland Development in the Mouse: Research Models and Techniques for Its Study from Past to Present

      • Jacqueline M. Veltmaat
      Pages 21-76

    3. Pubertal Mammary Gland Development: Elucidation of In Vivo Morphogenesis Using Murine Models

      • Jean McBryan, Jillian Howlin
      Pages 77-114

    4. Analysis of Mammary Gland Phenotypes by Transplantation of the Genetically Marked Mammary Epithelium

      • Duje Buric, Cathrin Brisken
      Pages 115-129

    5. Pubertal Ductal Morphogenesis: Isolation and Transcriptome Analysis of the Terminal End Bud

      • Joanna S. Morris, Torsten Stein
      Pages 131-148

    6. RNA Profiling of Non-cultured Fibroblasts Isolated from Pubertal Mouse Mammary Gland Sections

      • Ayman M. Ibrahim, Claire Cairney, Joanna S. Morris, Torsten Stein
      Pages 149-164

    7. Analysis of the Involuting Mouse Mammary Gland: An In Vivo Model for Cell Death

      • Bethan Lloyd-Lewis, Timothy J. Sargeant, Peter A. Kreuzaler, Henrike K. Resemann, Sara Pensa, Christine J. Watson
      Pages 165-186

  4. In Vitro 2D & 3D-Models

    1. Front Matter

      Pages 187-187
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    2. Contractility Assay for Established Myoepithelial Cell Lines

      • Stéphanie Cagnet, Marina A. Glukhova, Karine Raymond
      Pages 189-198

    3. Using 3D Culture of Primary Mammary Epithelial Cells to Define Molecular Entities Required for Acinus Formation: Analyzing MAP Kinase Phosphatases

      • Malgorzata Gajewska, Sara McNally
      Pages 199-216

    4. A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland

      • Zuzana Koledova, Pengfei Lu
      Pages 217-231

    5. Next Generation RNA Sequencing Analysis Reveals Expression of a Transient EMT Profile During Early Organization of MCF10A Cells in 3D

      • Benedikt Minke, Drieke Vandamme, Thomas Schwarzl, Walter Kolch, Finian Martin
      Pages 233-243

    6. A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells

      • Alexandra S. Piotrowski-Daspit, Allison K. Simi, Mei-Fong Pang, Joe Tien, Celeste M. Nelson
      Pages 245-257

  5. Mammary Stem Cells

    1. Front Matter

      Pages 259-259
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    2. Purification of Distinct Subsets of Epithelial Cells from Normal Human Breast Tissue

      • Mona Shehata, John Stingl
      Pages 261-276

    3. Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate

      • Gilbert H. Smith, Corinne A. Boulanger
      Pages 277-289

    4. Lineage Tracing of Mammary Stem and Progenitor Cells

      • Anoeska A. A. van de Moosdijk, Nai Yang Fu, Anne C. Rios, Jane E. Visvader, Renée van Amerongen
      Pages 291-308

  6. Translational Applications

    1. Front Matter

      Pages 309-309
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Keywords

About this book

This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols  will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists,  and those with an interest in molecular mechanisms underlying breast cancer.

Editors and Affiliations

  • School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Ireland

    Finian Martin

  • Institute of Cancer Sciences College of MVLS University of Glasgow, Glasgow, United Kingdom

    Torsten Stein

  • Division of Oncology-Pathology, Lund University Cancer Center/Medicon Village, Lund, Sweden

    Jillian Howlin

Bibliographic Information

  • Book Title: Mammary Gland Development

  • Book Subtitle: Methods and Protocols

  • Editors: Finian Martin, Torsten Stein, Jillian Howlin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6475-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6473-4Published: 29 October 2016

  • Softcover ISBN: 978-1-4939-8212-7Published: 10 July 2018

  • eBook ISBN: 978-1-4939-6475-8Published: 28 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 358

  • Number of Illustrations: 30 b/w illustrations, 32 illustrations in colour

  • Topics: Molecular Medicine

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