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High-Throughput Screening Assays in Toxicology pp 77–85Cite as

High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1473))

Abstract

Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.

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Authors and Affiliations

  1. National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA

    Sampada A. Shahane, Kana Nishihara & Menghang Xia

Authors
  1. Sampada A. Shahane
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  2. Kana Nishihara
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  3. Menghang Xia
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Corresponding author

Correspondence to Menghang Xia .

Editor information

Editors and Affiliations

  1. Department of Chemistry Rutgers Center for Computational and Integrative Biology, Rutgers University, Camden, New Jersey, USA

    Hao Zhu

  2. National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, Maryland, USA

    Menghang Xia

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Shahane, S.A., Nishihara, K., Xia, M. (2016). High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells. In: Zhu, H., Xia, M. (eds) High-Throughput Screening Assays in Toxicology. Methods in Molecular Biology, vol 1473. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6346-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6346-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6344-7

  • Online ISBN: 978-1-4939-6346-1

  • eBook Packages: Springer Protocols

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