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Protein Tyrosine Phosphatases

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Rafael Pulido

    1. BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain

    View editor publications

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  • Includes cutting-edge methods and protocols involving the essentials of PTP function and regulation
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1447)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. The Extended Family of Protein Tyrosine Phosphatases

    • Andrés Alonso, Caroline E. Nunes-Xavier, Yolanda Bayón, Rafael Pulido
    Pages 1-23

  3. Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome

    • Caroline E. Nunes-Xavier, Rafael Pulido
    Pages 25-37

  4. Expression, Purification, and Kinetic Analysis of PTP Domains

    • Mihaela Mentel, Rodica A. Badea, Georgiana Necula - Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, Stefan E. Szedlacsek
    Pages 39-66

  5. Peptide Microarrays for Real-Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples

    • Liesbeth Hovestad-Bijl, Jeroen van Ameijde, Dirk Pijnenburg, Riet Hilhorst, Rob Liskamp, Rob Ruijtenbeek
    Pages 67-78

  6. Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B

    • Sandra Luna, Janire Mingo, Olaia Aurtenetxe, Lorena Blanco, Laura Amo, Jan Schepens et al.
    Pages 79-93

  7. Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates

    • Laura Spinelli, Nicholas R. Leslie
    Pages 95-105

  8. Assessing the Biological Activity of the Glucan Phosphatase Laforin

    • Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz
    Pages 107-119

  9. Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents

    • Yunpeng Bai, Zhi-Hong Yu, Zhong-Yin Zhang
    Pages 121-138

  10. Analyzing Pseudophosphatase Function

    • Shantá D. Hinton
    Pages 139-153

  11. Crystallization of PTP Domains

    • Colin Levy, James Adams, Lydia Tabernero
    Pages 155-180

  12. NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases

    • Wolfgang Peti, Rebecca Page
    Pages 181-196

  13. Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs)

    • Christopher J. Caunt, Andrew M. Kidger, Stephen M. Keyse
    Pages 197-215

  14. In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions

    • Sina Koch, Irene Helbing, Sylvia-Annette Böhmer, Makoto Hayashi, Lena Claesson-Welsh, Ola Söderberg et al.
    Pages 217-242

  15. Use of Dominant-Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates

    • Vegesna Radha
    Pages 243-265

  16. Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains

    • Andrew William Stoker
    Pages 267-281

  17. Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling

    • Eynat Finkelshtein, Einat Levy-Apter, Ari Elson
    Pages 283-300

  18. Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Hemostasis

    • Souad Rahmouni, Alexandre Hego, Céline Delierneux, Odile Wéra, Lucia Musumeci, Lutz Tautz et al.
    Pages 301-330

  19. Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis

    • Mathieu Amand, Charlotte Erpicum, Christine Gilles, Agnès Noël, Souad Rahmouni
    Pages 331-349

  20. Studying Protein-Tyrosine Phosphatases in Zebrafish

    • Alexander James Hale, Jeroen den Hertog
    Pages 351-372
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Keywords

About this book

This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Tyrosine Phosphatases: Methodsand Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.

Editors and Affiliations

  • BioCruces Health Research Institute, Barakaldo, Bizkaia, Spain

    Rafael Pulido

Bibliographic Information

  • Book Title: Protein Tyrosine Phosphatases

  • Book Subtitle: Methods and Protocols

  • Editors: Rafael Pulido

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3746-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3744-8Published: 12 August 2016

  • Softcover ISBN: 978-1-4939-8127-4Published: 09 June 2018

  • eBook ISBN: 978-1-4939-3746-2Published: 11 August 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 402

  • Number of Illustrations: 28 b/w illustrations, 51 illustrations in colour

  • Topics: Protein Science

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