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Molecular Genetics of Asthma

  • Book
  • © 2016

Overview

Editors:
  1. María Isidoro García

    1. Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain

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  • Includes cutting-edge methods and protocols involving the molecular genetics of asthma
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1434)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Applications of Molecular Genetics to the Study of Asthma

    • Catalina S. Sanz-Lozano, Virginia García-Solaesa, Ignacio Davila, María Isidoro-García
    Pages 1-13

  3. Assessment and Validation of New Genetic Variants: A Systematic In Silico Approach

    • Belén García-Berrocal, Asunción García-Sánchez, María Isidoro-García
    Pages 15-27

  4. Introduction to the Gene Expression Analysis

    • Ignacio San Segundo-Val, Catalina S. Sanz-Lozano
    Pages 29-43

  5. Real-Time PCR for Gene Expression Quantification in Asthma

    • Ignacio San Segundo-Val, Virginia García-Solaesa, Asunción García-Sánchez
    Pages 45-55

  6. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma

    • Ignacio San Segundo-Val, Catalina S. Sanz-Lozano
    Pages 57-69

  7. Review of Methods to Study Gene Expression Regulation Applied to Asthma

    • Asunción García-Sánchez, Fernando Marqués-García
    Pages 71-89

  8. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma

    • Virginia García-Solaesa, Catalina S. Sanz-Lozano
    Pages 91-105

  9. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma

    • Virginia García-Solaesa, Sara Ciria Abad
    Pages 107-120

  10. Chromatin Immunoprecipitation: Application to the Study of Asthma

    • Asunción García-Sánchez, Fernándo Marqués-García
    Pages 121-137

  11. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols

    • Asunción García-Sánchez, Fernando Marqués-García
    Pages 139-151

  12. Protocols for Exosome Isolation and RNA Profiling

    • Fernando Marqués-García, María Isidoro-García
    Pages 153-167

  13. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell

    • Elena Marcos-Vadillo, Asunción García-Sánchez
    Pages 169-183

  14. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line

    • Elena Marcos-Vadillo, Asunción García-Sánchez
    Pages 185-197

  15. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line

    • Elena Marcos-Vadillo, Asunción García-Sánchez
    Pages 199-211

  16. Review of Mouse Models Applied to the Study of Asthma

    • Fernando Marqués-García, Elena Marcos-Vadillo
    Pages 213-222

  17. General and Specific Mouse Models for Asthma Research

    • Fernando Marqués-García, Elena Marcos-Vadillo
    Pages 223-237

  18. Mouse Models Applied to the Research of Pharmacological Treatments in Asthma

    • Fernando Marqués-García, Elena Marcos-Vadillo
    Pages 239-253

  19. Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma

    • Almudena Sánchez-Martín, Asunción García-Sánchez, María Isidoro-García
    Pages 255-272

  20. Back Matter

    Pages 273-275
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Keywords

About this book

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.  Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.

Editors and Affiliations

  • Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain

    María Isidoro García

Bibliographic Information

  • Book Title: Molecular Genetics of Asthma

  • Editors: María Isidoro García

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3652-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3650-2Published: 15 June 2016

  • Softcover ISBN: 978-1-4939-8107-6Published: 30 May 2018

  • eBook ISBN: 978-1-4939-3652-6Published: 14 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 275

  • Number of Illustrations: 5 b/w illustrations, 38 illustrations in colour

  • Topics: Molecular Medicine

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