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  • © 2016

Forensic DNA Typing Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1420)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
  2. Collection of Samples for DNA Analysis

    • Roland A. H. van Oorschot, Timothy J. Verdon, Kaye N. Ballantyne
    Pages 1-12
  3. Body Fluid Identification Using mRNA Profiling

    • Amy D. Roeder, Cordula Haas
    Pages 13-31
  4. mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification

    • Joanna Jakubowska, Agnieszka Maciejewska, Ryszard Pawłowski
    Pages 33-42
  5. DNA Extraction: Organic and Solid-Phase

    • Wafa Altayari
    Pages 55-68
  6. Extraction of DNA from Skeletal Remains

    • Suni M. Edson, Timothy P. McMahon
    Pages 69-87
  7. Extraction of DNA from Human Skeletal Material

    • Irena Zupanič Pajnič
    Pages 89-108
  8. Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex® Kit

    • Majid Bashir, Nur Haliza Bt Hassan
    Pages 135-142
  9. Whole Human Mitochondrial DNA Sequencing

    • Igor V. Ovchinnikov, Mathew J. Malek, Katelyn Kjelland, Kenneth Drees
    Pages 157-171
  10. In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome

    • B. Bekaert, R. Ellerington, L. Van den Abbeele, R. Decorte
    Pages 173-183
  11. Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry-Informative Marker Sets

    • Chris Phillips, Carla Santos, Manuel Fondevila, Ángel Carracedo, Maria Victoria Lareu
    Pages 233-253
  12. Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data

    • Carla Santos, Chris Phillips, A. Gomez-Tato, J. Alvarez-Dios, Ángel Carracedo, Maria Victoria Lareu
    Pages 255-285

About this book

This volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science.

 

Reviews

“This is an instructional book that raises awareness of the technology available in genetic forensic sciences. … The book is written in a way that is easy to understand for advanced professionals as well as beginners. Anyone wanting to understand the technology involved in forensic DNA analysis would benefit from this unique book. … The value of this book lies in the simple and logical way it presents complex information in forensic medicine.” (Luis F. Escobar, Doody's Book Reviews, November, 2016)

Editors and Affiliations

  • School of Forensic and Applied Sciences, University of Central Lancashire, Preston, United Kingdom

    William Goodwin

Bibliographic Information

  • Book Title: Forensic DNA Typing Protocols

  • Editors: William Goodwin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3597-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3595-6Published: 04 June 2016

  • Softcover ISBN: 978-1-4939-8089-5Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3597-0Published: 03 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 298

  • Number of Illustrations: 10 b/w illustrations, 18 illustrations in colour

  • Topics: Human Genetics, Forensic Medicine

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access