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Advanced Patch-Clamp Analysis for Neuroscientists

  • Book
  • © 2016

Overview

Editors:
  1. Alon Korngreen

    1. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 113)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. In Vivo Whole-Cell Recordings

    • Bojana Kokinovic, Stylianos Papaioannou, Paolo Medini
    Pages 1-19

  3. Juxtasomal Loose-Patch Recordings in Awake, Head-Fixed Rats to Study the Link Between Structure and Function of Individual Neurons

    • Christiaan P. J. de Kock
    Pages 21-35

  4. Studying Sodium Channel Gating in Heterologous Expression Systems

    • Jannis E. Meents, Angelika Lampert
    Pages 37-65

  5. Elucidating the Link Between Structure and Function of Ion Channels and Transporters with Voltage-Clamp and Patch-Clamp Fluorometry

    • Giovanni Zifarelli, Jana Kusch
    Pages 67-95

  6. Dendrites: Recording from Fine Neuronal Structures Using Patch-Clamp and Imaging Techniques

    • Sonia Gasparini, Lucy M. Palmer
    Pages 97-121

  7. Patch-Clamp Recording from Myelinated Central Axons

    • Maarten H. P. Kole, Marko A. Popovic
    Pages 123-138

  8. Analysis of Transsynaptic Attentional Neuronal Circuits with Octuple Patch-Clamp Recordings

    • Daniel R. Wyskiel, Trevor C. Larry, Xiaolong Jiang, Guangfu Wang, J. Julius Zhu
    Pages 139-150

  9. Optogenetic Dissection of the Striatal Microcircuitry

    • Gilad Silberberg, Henrike Planert
    Pages 151-170

  10. Paired Recordings from Synaptically Coupled Neurones in Acute Neocortical Slices

    • Dirk Feldmeyer, Gabriele Radnikow
    Pages 171-191

  11. Extracting Quantal Properties of Transmission at Central Synapses

    • Frederic Lanore, R. Angus Silver
    Pages 193-211

  12. Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo

    • Balázs Rózsa, Gergely Szalay, Gergely Katona
    Pages 213-245

  13. Intracellular Voltage-Sensitive Dyes for Studying Dendritic Excitability and Synaptic Integration

    • Corey D. Acker, Mandakini B. Singh, Srdjan D. Antic
    Pages 247-265

  14. Modeling the Kinetic Mechanisms of Voltage-Gated Ion Channels

    • Autoosa Salari, Marco A. Navarro, Lorin S. Milescu
    Pages 267-304

  15. Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches

    • Mara Almog, Alon Korngreen
    Pages 305-323

  16. Creating and Constraining Compartmental Models of Neurons Using Experimental Data

    • Stefanos S. Stefanou, George Kastellakis, Panayiota Poirazi
    Pages 325-343

  17. Back Matter

    Pages 345-350
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Keywords

About this book

This volume presents current adaptations of the patch-clamp technique to neuroscience. Chapters focus on in-vivo recordings, voltage-gated channel recording and analysis, dendritic and axonal recordings, synaptic current recording and analysis, advanced fluorescent techniques, optogenetics and voltage-sensitive dye imaging, and finally channel and neuronal modeling. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Advanced Patch-Clamp Analysis for Neuroscientists aims to ensure successful results in the further study of this vital field.

Reviews

“This an excellent text and atlas on patch clamping protocol of single neurons, axons, and dendrites from a neurophysiologist’s viewpoint. … Color morphology is nicely illustrated. … I recommend this book for both beginners and advanced scientists.” (Joseph J. Grenier, Amazon.com, February, 2016)

Editors and Affiliations

  • The Leslie and Susan Gonda Multidisciplinary Brain Research Center, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    Alon Korngreen

Bibliographic Information

  • Book Title: Advanced Patch-Clamp Analysis for Neuroscientists

  • Editors: Alon Korngreen

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-3411-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3409-6Published: 15 January 2016

  • Softcover ISBN: 978-1-4939-8043-7Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3411-9Published: 14 January 2016

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 350

  • Number of Illustrations: 53 b/w illustrations, 28 illustrations in colour

  • Topics: Neurosciences

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