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  • Book
  • © 2016

Clinical Applications of PCR

  • Includes fully updated, cutting-edge methods and protocols for PCR in a clinical setting
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1392)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. COLD-PCR: Applications and Advantages

    • Zhuang Zuo, Kausar J. Jabbar
    Pages 17-25
  3. PCR-Based Detection of DNA Copy Number Variation

    • Meenakshi Mehrotra
    Pages 27-32
  4. Emulsion PCR: Techniques and Applications

    • Rashmi Kanagal-Shamanna
    Pages 33-42
  5. Digital PCR: Principles and Applications

    • Rashmi Kanagal-Shamanna
    Pages 43-50
  6. High-Resolution Melt Curve Analysis in Cancer Mutation Screen

    • Meenakshi Mehrotra, Keyur P. Patel
    Pages 63-69
  7. Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach

    • Eglys González-Marcano, Hirotomo Kato, Juan Luis Concepción, María Elizabeth Márquez, Alberto Paniz Mondolfi
    Pages 113-124
  8. Detection of Trypanosoma cruzi by Polymerase Chain Reaction

    • María Elizabeth Márquez, Juan Luis Concepción, Eglys González-Marcano, Alberto Paniz Mondolfi
    Pages 125-141
  9. PCR Techniques in Next-Generation Sequencing

    • Rashmi S. Goswami
    Pages 143-151
  10. Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics

    • Chi Young Ok, Rajesh R. Singh, Alaa A. Salim
    Pages 153-159
  11. Quantitative Real-Time PCR: Recent Advances

    • Charanjeet Singh, Sinchita Roy-Chowdhuri
    Pages 161-176
  12. PCR Techniques in Characterizing DNA Methylation

    • Khalida Wani, Kenneth D. Aldape
    Pages 177-186
  13. Back Matter

    Pages 187-191

About this book

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.

Reviews

“This multi-author treatise reviews the clinical applications of PCR in various fields of medicine stressing its importance for genetic analysis. It has 16 chapters, each with color illustrations and references. Recommended for researchers in biology and hospital laboratories.” (Pediatric Endocrinology Reviews (PER), Vol. 14 (3), March, 2017)

Editors and Affiliations

  • Anderson Cancer Center, The University of Texas MD, Houston, USA

    Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

Bibliographic Information

  • Book Title: Clinical Applications of PCR

  • Editors: Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3360-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3358-7Published: 03 February 2016

  • Softcover ISBN: 978-1-4939-8030-7Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3360-0Published: 03 February 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 191

  • Number of Illustrations: 9 b/w illustrations, 16 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access