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Advances and Clinical Practice in Pyrosequencing

  • Book
  • © 2016

Overview

Editors:
  1. Guohua Zhou

    1. State Key Laboratory of Analytical Chemi, Medical School of Nanjing University, Nanjing, China

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    You can also search for this editor in PubMed   Google Scholar

  2. Qinxin Song

    1. Key Laboratory of Drug Quality Control, China Pharmaceutical University, Nanjing, China

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides a comprehensive description of current techniques and clinical applications of pyrosequencing
  • Addresses both the technical and clinical aspects of pyrosequencing uses
  • Describes the current applications of pyrosequencing in different fields, emphasizing their practicality and utility in clinical treatment, especially gene-guided personalized medicine
  • Provides technical protocols to measure SNPs or mutations, mRNA and microRNA quantification, as well as resequencing by pyrosequencing
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Technology Preparation

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Preparation of Single-Stranded DNA for Pyrosequencing by LATE-PCR

      • Huiyong Yang, Chao Liang, Zhiyao Chen, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 3-12

    3. A Simplified Protocol for Preparing Pyrosequencing Templates Based on LATE-PCR Using Whole Blood as Starting Material Directly

      • Yunlong Liu, Haiping Wu, Hui Ye, Zhiyao Chen, Qinxin Song, Bingjie Zou et al.
      Pages 13-21

    4. Improvement of LATE-PCR to Prepare Pyrosequencing Template

      • Qinxin Song, Huiyong Yang, Bingjie Zou, Hideki Kambara, Guohua Zhou
      Pages 23-30

    5. Pyrosequencing Templates Generated by Nicking PCR Products with Nicking Endonuclease

      • Qinxin Song, Haiping Wu, Guohua Zhou, Hideki Kambara
      Pages 31-39

    6. Pyrosequencing Templates Generated by Asymmetric Nucleic Acid Sequence-Based Amplification (Asymmetric-NASBA)

      • Huning Jia, Zhiyao Chen, Haiping Wu, Hui Ye, Bingjie Zou, Qinxin Song et al.
      Pages 41-49

    7. Gel Immobilization of Acrylamide-Modified Single-Stranded DNA Template for Pyrosequencing

      • Pengfeng Xiao, Huan Huang, Bingjie Zou, Qinxin Song, Guohua Zhou, Zuhong Lu
      Pages 51-65

    8. Multiplex PCR Based on a Universal Biotinylated Primer to Generate Templates for Pyrosequencing

      • Zhiyao Chen, Yunlong Liu, Hui Ye, Haiping Wu, Jinheng Li, Bingjie Zou et al.
      Pages 67-76

  3. Template Innovation

    1. Front Matter

      Pages 77-77
      Download chapter PDF

    2. A Novel Pyrosequencing Principle Based on AMP–PPDK Reaction for Improving the Detection Limit

      • Guohua Zhou, Tomoharu Kajiyama, Mari Gotou, Akihiko Kishimoto, Shigeya Suzuki, Hideki Kambara
      Pages 79-94

    3. Pyrosequencing Chemistry Coupled with Modified Primer Extension Reactions for Quantitative Detection of Allele Frequency in a Pooled Sample

      • Guohua Zhou, Masao Kamahori, Kazunori Okano, Gao Chuan, Kunio Harada, Hideki Kambara
      Pages 95-114

    4. A Gel-Free SNP Genotyping Method Based on Pyrosequencing Chemistry Coupled with Modified Primer Extension Reactions Directly from Double-Stranded PCR Products

      • Guohua Zhou, Hiromi Shirakura, Masao Kamahori, Kazunori Okano, Keiichi Nagai, Hideki Kambara
      Pages 115-128

    5. Improvement of Pyrosequencing to Allow Multiplex SNP Typing in a Pyrogram

      • Guohua Zhou, Mari Gotou, Tomoharu Kajiyama, Hideki Kambara
      Pages 129-143

    6. Improvement of Pyrosequencing Sensitivity by Capturing Free Adenosine 5′-Phosphosulfate with Adenosine Triphosphate Sulfurylase

      • Haiping Wu, Wenjuan Wu, Zhiyao Chen, Weipeng Wang, Bingjie Zou, Qinxin Song et al.
      Pages 145-154

    7. Using Polymerase Preference Index to Design imLATE-PCR Primers for an Efficient Pyrosequencing

      • Qinxin Song, Xiemin Qi, Yiyun Shen, Yiru Jin, Bingjie Zou, Guohua Zhou
      Pages 155-166

  4. Reagents Preparation

    1. Front Matter

      Pages 167-167
      Download chapter PDF

    2. Preparation of Thermal-Stable Biotinylated Firefly Luciferase and Its Application in Pyrosequencing

      • Shuhui Zhu, Bingjie Zou, Haiping Wu, Yinjiao Ma, Ying Chen, Qinxin Song et al.
      Pages 169-177

    3. Expression of Thermostable Recombinant Luciola lateralis Luciferase and Its Function on Setting Up of Heat-Stable Pyrosequencing System

      • Shu Xu, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 179-185

    4. Expression and Purification of ATP Sulfurylase from Saccharomyces cerevisias in Escherichia coli and Its Application in Pyrosequencing

      • Juan Luo, Wenjuan Wu, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 187-195
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Keywords

About this book

The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology.  Divided into five parts, the book’s thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab.

Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields.

Editors and Affiliations

  • State Key Laboratory of Analytical Chemi, Medical School of Nanjing University, Nanjing, China

    Guohua Zhou

  • Key Laboratory of Drug Quality Control, China Pharmaceutical University, Nanjing, China

    Qinxin Song

Bibliographic Information

  • Book Title: Advances and Clinical Practice in Pyrosequencing

  • Editors: Guohua Zhou, Qinxin Song

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-4939-3308-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3306-8Published: 05 February 2016

  • Softcover ISBN: 978-1-4939-8017-8Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3308-2Published: 04 February 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XIV, 407

  • Number of Illustrations: 44 b/w illustrations, 112 illustrations in colour

  • Topics: Human Genetics

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