Skip to main content

Application of Aptamers in Histopathology

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1380))

Abstract

Aptamers are proving to be exceedingly effective in a number of applications. Given the disadvantages of antibodies, such as batch-to-batch variation and cross-reactivity, aptamers have the potential to revolutionize the field of histopathology due to their high specificity and the ease of their synthesis and modification. Here, we describe a chromogenic staining method for paraffin-embedded tissue sections with FITC-labeled aptamers.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Bordeaux J, Welsh A, Agarwal S, Killiam E, Baquero M, Hanna J et al (2010) Antibody validation. Biotechniques 48:197–209

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Perkel JM (2014) The antibody challenge. Biotechniques 56:111–114

    CAS  PubMed  Google Scholar 

  3. Ellington AD, Szostak JW (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346:818–822

    Article  CAS  PubMed  Google Scholar 

  4. Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249:505–510

    Article  CAS  PubMed  Google Scholar 

  5. Shigdar S, Lin J, Yu Y, Pastuovic M, Wei M, Duan W (2011) RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci 102:991–998

    Article  CAS  PubMed  Google Scholar 

  6. Shigdar S, Qiao L, Zhou SF, Xiang D, Wang T, Li Y et al (2013) RNA aptamers targeting cancer stem cell marker CD133. Cancer Lett 330:84–95

    Article  CAS  PubMed  Google Scholar 

  7. Zeng Z, Zhang P, Zhao N, Sheehan AM, Tung CH, Chang CC et al (2010) Using oligonucleotide aptamer probes for immunostaining of formalin-fixed and paraffin-embedded tissues. Mod Pathol 23:1553–1558

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Gupta S, Thirstrup D, Jarvis TC, Schneider DJ, Wilcox SK, Carter J et al (2011) Rapid histochemistry using slow off-rate modified aptamers with anionic competition. Appl Immunohistochem Mol Morphol 19:273–278

    PubMed  Google Scholar 

  9. Shigdar S, Qian C, Lv L, Pu C, Li Y, Li L et al (2013) The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One 8, e57613

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A 76:3683–3687

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. van Gijlswijk RP, Wiegant J, Raap AK, Tanke HJ (1996) Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol. J Histochem Cytochem 44:389–392

    Article  PubMed  Google Scholar 

  12. Singh L, Jones KW (1984) The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures. Nucleic Acids Res 12:5627–5638

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Song Y, Zhu Z, An Y, Zhang W, Zhang H, Liu D et al (2013) Selection of DNA aptamers against epithelial cell adhesion molecule for cancer cell imaging and circulating tumor cell capture. Anal Chem 85:4141–4149

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Lifen Wang or Wei Duan .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Shigdar, S., Lv, L., Wang, L., Duan, W. (2016). Application of Aptamers in Histopathology. In: Mayer, G. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 1380. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3197-2_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3197-2_16

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3196-5

  • Online ISBN: 978-1-4939-3197-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics