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Mitochondrial DNA

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Matthew McKenzie

    1. Hudson Institute of Medical Research, Clayton, Melbourne, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1351)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Detection of Mitochondrial DNA Mutations and Mitochondrial DNA Damage

    1. Front Matter

      Pages 1-1
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    2. Comprehensive Mitochondrial Genome Analysis by Massively Parallel Sequencing

      • Meagan E. Palculict, Victor Wei Zhang, Lee-Jun Wong, Jing Wang
      Pages 3-17

    3. Analysis of Translesion DNA Synthesis by the Mitochondrial DNA Polymerase γ

      • William C. Copeland, Rajesh Kasiviswanathan, Matthew J. Longley
      Pages 19-26

    4. Quantification of DNA Damage by Real-Time qPCR

      • Wei Wang, Katja Scheffler, Ying Esbensen, Lars Eide
      Pages 27-32

    5. Quantitation of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single-Molecule PCR

      • Yevgenya Kraytsberg, Xinhong Guo, Saisai Tao, Alexandra Kuznetsov, Catherine MacLean, Daniel Ehrlich et al.
      Pages 33-46

  3. Visualization of Mitochondrial DNA and Mitochondrial DNA Nucleoids

    1. Front Matter

      Pages 47-47
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    2. A Single-Cell Resolution Imaging Protocol of Mitochondrial DNA Dynamics in Physiopathology, mTRIP, Which Also Evaluates Sublethal Cytotoxicity

      • Laurent Chatre, Benjamin Montagne, Miria Ricchetti
      Pages 49-65

    3. Scalable Isolation of Mammalian Mitochondria for Nucleic Acid and Nucleoid Analysis

      • Ken-Wing Lee, Daniel F. Bogenhagen
      Pages 67-79

    4. Tracking Mitochondrial DNA In Situ

      • Anna Ligasová, Karel Koberna
      Pages 81-92

  4. Analysis of Mitochondrial DNA Replication, Mitochondrial DNA-Encoded Protein Translation and Mitochondrial DNA Copy Number

    1. Front Matter

      Pages 93-93
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    2. Analysis of Replicating Mitochondrial DNA by In Organello Labeling and Two-Dimensional Agarose Gel Electrophoresis

      • Ian J. Holt, Lawrence Kazak, Aurelio Reyes, Stuart R. Wood
      Pages 95-113

    3. Translation and Assembly of Radiolabeled Mitochondrial DNA-Encoded Protein Subunits from Cultured Cells and Isolated Mitochondria

      • Luke E. Formosa, Annette Hofer, Christin Tischner, Tina Wenz, Michael T. Ryan
      Pages 115-129

    4. Analysis of Mitochondrial DNA Copy Number and Its Regulation Through DNA Methylation of POLGA

      • Xin Sun, William Lee, Vijesh Vaghjiani, Justin C. St. John
      Pages 131-141

  5. Modification of Mitochondrial DNA

    1. Front Matter

      Pages 143-143
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    2. Engineered mtZFNs for Manipulation of Human Mitochondrial DNA Heteroplasmy

      • Payam A. Gammage, Lindsey Van Haute, Michal Minczuk
      Pages 145-162

    3. Generation of Xenomitochondrial Embryonic Stem Cells for the Production of Live Xenomitochondrial Mice

      • Ian A. Trounce, Jessica Ackerley, Matthew McKenzie
      Pages 163-173

    4. Import of Fluorescent RNA into Mitochondria of Living Cells

      • Jaroslav Zelenka, Petr Ježek
      Pages 175-181

  6. Purification of Proteins Involved in Mitochondrial DNA Replication and Transcription

    1. Front Matter

      Pages 183-183
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    2. Purification and Comparative Assay of the Human Mitochondrial Replicative DNA Helicase

      • Fernando A. Rosado-Ruiz, Minyoung So, Laurie S. Kaguni
      Pages 185-198
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Keywords

About this book

This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and  mitochondrial DNA copy number,  the latest technologies for modifying the mitochondrial genome and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.

Editors and Affiliations

  • Hudson Institute of Medical Research, Clayton, Melbourne, Australia

    Matthew McKenzie

Bibliographic Information

  • Book Title: Mitochondrial DNA

  • Book Subtitle: Methods and Protocols

  • Editors: Matthew McKenzie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3040-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3039-5Published: 07 November 2015

  • Softcover ISBN: 978-1-4939-4944-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3040-1Published: 03 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 234

  • Topics: Human Genetics

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