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Embryonic Stem Cell Protocols

Editors:

  • Includes cutting-edge methods and protocols in embryonic stem cell research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1341)

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Hardcover Book USD 169.99
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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
  2. A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

    • Keitaro Imaizumi, Momoe Iha, Naoki Nishishita, Shin Kawamata, Shinichi Nishikawa, Teruo Akuta
    Pages 15-24
  3. Generation of Embryonic Stem Cells in Rabbits

    • Marielle Afanassieff, Pierre Osteil, Pierre Savatier
    Pages 49-66
  4. Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction

    • Ortwin Naujok, Ulf Diekmann, Matthias Elsner
    Pages 67-85
  5. Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells

    • Anna Pezzarossa, Ana M. V. Guedes, Domingos Henrique, Elsa Abranches
    Pages 87-100
  6. Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis

    • James Chappell, Ben Boward, Stephen Dalton
    Pages 101-110
  7. Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells

    • Lida Radan, Chris S. Hughes, Jonathan H. Teichroeb, Lynne-Marie Postovit, Dean H. Betts
    Pages 133-142
  8. Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells

    • Anisa B. Rahim, Leah A. Vardy
    Pages 143-155
  9. Intracellular Calcium Measurements for Functional Characterization of Neuronal Phenotypes

    • Talita Glaser, Ana Regina G. Castillo, Ágatha Oliveira, Henning Ulrich
    Pages 245-255

About this book

This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to move very quickly, thus the kinds of studies continue to expand and diversify, and methodologies are continuously being refined and improved, which this book reflects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and fully updated, Embryonic Stem Cell Protocols, Third Edition serves as an ideal reference for researchers investigating this rich area of study.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Embryonic Stem Cell Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2954-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2953-5Published: 02 December 2015

  • Softcover ISBN: 978-1-4939-7976-9Published: 29 March 2019

  • eBook ISBN: 978-1-4939-2954-2Published: 30 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 449

  • Number of Illustrations: 35 b/w illustrations, 49 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access