Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1329)
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Table of contents (23 protocols)
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Front Matter
About this book
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Editors and Affiliations
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National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Laboratory of Molecular Biology, , Bethesda, USA
Susan Buchanan
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Department of Biological Sciences,, Markey Center for Structural Biology, Purdue University, West Lafayette, USA
Nicholas Noinaj
Bibliographic Information
Book Title: The BAM Complex
Book Subtitle: Methods and Protocols
Editors: Susan Buchanan, Nicholas Noinaj
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2871-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2870-5Published: 02 October 2015
Softcover ISBN: 978-1-4939-4920-5Published: 23 August 2016
eBook ISBN: 978-1-4939-2871-2Published: 01 October 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 283
Number of Illustrations: 27 b/w illustrations, 16 illustrations in colour
Topics: Protein Science, Membrane Biology