Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1311)
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Table of contents (23 protocols)
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Front Matter
About this book
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
Editors and Affiliations
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Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden
Magnus Lundgren
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Department of Molecular Biology MIMS, Umeå University, Umeå, Sweden
Emmanuelle Charpentier
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Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand
Peter C. Fineran
Bibliographic Information
Book Title: CRISPR
Book Subtitle: Methods and Protocols
Editors: Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2687-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2686-2Published: 20 May 2015
Softcover ISBN: 978-1-4939-5428-5Published: 09 October 2016
eBook ISBN: 978-1-4939-2687-9Published: 18 May 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 366
Number of Illustrations: 26 b/w illustrations, 37 illustrations in colour
Topics: Gene Expression