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NF-kappa B

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Michael J. May

    1. Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1280)

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Table of contents (38 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Standard Approaches to Detect NF-κB Pathway Activation

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Electrophoretic Mobility Shift Assay Analysis of NF-κB DNA Binding

      • Sitharam Ramaswami, Matthew S. Hayden
      Pages 3-13

    3. Detection of IκB Degradation Dynamics and IκB-α Ubiquitination

      • Petro Starokadomskyy, Ezra Burstein
      Pages 15-24

    4. Measurement of NF-κB Transcriptional Activity and Identification of NF-κB cis-Regulatory Elements Using Luciferase Assays

      • Patricia E. Collins, Christine O’Carroll, Ruaidhrí J. Carmody
      Pages 25-43

  3. Detection and Analysis of NF-κB Signaling

    1. Front Matter

      Pages 45-45
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    2. Assessing Sites of NF-κB DNA Binding Using Chromatin Immunoprecipitation

      • Amy Colleran, Patricia E. Collins, Ruaidhrí J. Carmody
      Pages 47-59

    3. IKK Kinase Assay for Assessment of Canonical NF-κB Activation in Neurons

      • Anca B. Mihalas, Mollie K. Meffert
      Pages 61-74

    4. Analysis of Epidermal Growth Factor-Induced NF-κB Signaling

      • Changying Jiang, Xin Lin
      Pages 75-102

    5. Methods to Assess the Activation of the Alternative (Noncanonical) NF-κB Pathway by Non-death TNF Receptors

      • Caroline Remouchamps, Emmanuel Dejardin
      Pages 103-119

    6. Systematic Detection of Noncanonical NF-κB Activation

      • Zhaoxia Qu, Gutian Xiao
      Pages 121-154

    7. Noncanonical NF-κB Activation and SDF-1 Expression in Human Endothelial Cells

      • Kelly A. McCorkell, Michael J. May
      Pages 155-180

    8. Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblasts

      • Carolyn M. Gray, Michael J. May
      Pages 181-195

    9. Dissecting NF-κB Signaling Induced by Genotoxic Agents via Genetic Complementation of NEMO-Deficient 1.3E2 Cells

      • Shawn S. Jackson, Shigeki Miyamoto
      Pages 197-215

  4. Methods to Study the Control of NF-κB Signaling

    1. Front Matter

      Pages 217-217
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    2. Visualizing TCR-Induced POLKADOTS Formation and NF-κB Activation in the D10 T-Cell Clone and Mouse Primary Effector T Cells

      • Suman Paul, Brian C. Schaefer
      Pages 219-238

    3. Detection of Recombinant and Cellular MALT1 Paracaspase Activity

      • Daniel Nagel, Daniel Krappmann
      Pages 239-246

    4. TRAF Protein Function in Noncanonical NF-κB Signaling

      • Anna D. Reichardt, Jose Pindado, Shivam A. Zaver, Genhong Cheng
      Pages 247-268

    5. Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-κB Signaling

      • Eugene Varfolomeev, Tatiana Goncharov, Domagoj Vucic
      Pages 269-282

    6. Elucidating Dynamic Protein–Protein Interactions and Ubiquitination in NF-κB Signaling Pathways

      • Noula Shembade, Edward W. Harhaj
      Pages 283-295
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Keywords

About this book

Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.

Editors and Affiliations

  • Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, USA

    Michael J. May

Bibliographic Information

  • Book Title: NF-kappa B

  • Book Subtitle: Methods and Protocols

  • Editors: Michael J. May

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2422-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2421-9Published: 04 March 2015

  • Softcover ISBN: 978-1-4939-4811-6Published: 09 October 2016

  • eBook ISBN: 978-1-4939-2422-6Published: 03 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 668

  • Number of Illustrations: 56 b/w illustrations, 61 illustrations in colour

  • Topics: Biochemistry, general, Posttranslational Modification

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