Skip to main content
  • Book
  • © 2015

RNA Remodeling Proteins

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproductible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1259)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Happy Birthday: 25 Years of DEAD-Box Proteins

    • Patrick Linder, Frances Fuller-Pace
    Pages 17-33
  3. Dynamics of the Spb4 Interactome Monitored by Affinity Purification

    • Juan José García-Gómez, Simon Lebaron, Yves Henry, Jesús de la Cruz
    Pages 49-67
  4. Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU

    • Evan P. Booy, Ewan K. S. McRae, Sean A. McKenna
    Pages 125-135
  5. Measuring Helicase Inhibition of the DEAD-Box Protein Dbp2 by Yra1

    • Wai Kit Ma, Elizabeth J. Tran
    Pages 183-197
  6. A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism

    • Atsushi Furuta, Kazi Abdus Salam, Hidenori Tani, Satoshi Tsuneda, Yuji Sekiguchi, Nobuyoshi Akimitsu et al.
    Pages 211-228
  7. Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-Molecule Level

    • Desiree Salas, Veronika Gocheva, Marcelo Nöllmann
    Pages 257-273
  8. Probing RNA Translocases with DNA

    • Kimberly A. Reynolds, Veronica M. Raney, Kevin D. Raney
    Pages 275-291
  9. Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis

    • François D’Heygère, Annie Schwartz, Franck Coste, Bertrand Castaing, Marc Boudvillain
    Pages 293-311

About this book

This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, RNA Remodeling Proteins: Methods and Protocols seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.

Editors and Affiliations

  • CNRS Centre de Biophysique Moléculaire, Orléans, France

    Marc Boudvillain

Bibliographic Information

  • Book Title: RNA Remodeling Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Marc Boudvillain

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2214-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2213-0Published: 13 January 2015

  • Softcover ISBN: 978-1-4939-4899-4Published: 24 September 2016

  • eBook ISBN: 978-1-4939-2214-7Published: 12 January 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 470

  • Number of Illustrations: 44 b/w illustrations, 40 illustrations in colour

  • Topics: Protein Science, Protein Structure

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access