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  • © 2015

Polyadenylation in Plants

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1255)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-ix
  2. Bioinformatics Studies of Plant Polyadenylation

    1. Front Matter

      Pages 1-1
    2. Computational Analysis of Plant Polyadenylation Signals

      • Xiaohui Wu, Guoli Ji, Qingshun Quinn Li
      Pages 3-11
    3. Prediction of Plant mRNA Polyadenylation Sites

      • Xiaohui Wu, Guoli Ji, Qingshun Quinn Li
      Pages 13-23
    4. Extraction of Poly(A) Sites from Large-Scale RNA-seq Data

      • Min Dong, Guoli Ji, Qingshun Quinn Li, Chun Liang
      Pages 25-37
    5. Poly(A)-Tag Deep Sequencing Data Processing to Extract Poly(A) Sites

      • Xiaohui Wu, Guoli Ji, Qingshun Quinn Li
      Pages 39-48
  3. Biochemistry of Polyadenylation in Plants

    1. Front Matter

      Pages 67-67
    2. In Vitro Analysis of Cleavage and Polyadenylation in Arabidopsis

      • Hongwei Zhao, Qingshun Quinn Li
      Pages 79-89
    3. A 3′ RACE Protocol to Confirm Polyadenylation Sites

      • Liuyin Ma, Arthur G. Hunt
      Pages 135-144
  4. Genome-Scale Study of Polyadenylation in Plants

    1. Front Matter

      Pages 145-145
    2. Phage Display Library Screening for Identification of Interacting Protein Partners

      • Balasubrahmanyam Addepalli, Suryadevara Rao, Arthur G. Hunt
      Pages 147-158
    3. Genome-Wide Determination of Poly(A) Site Choice in Plants

      • Pratap Kumar Pati, Liuyin Ma, Arthur G. Hunt
      Pages 159-174
    4. Poly(A) Tag Library Construction from 10 ng Total RNA

      • Jingyi Cao, Qingshun Quinn Li
      Pages 185-194

About this book

This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Some are specific for plant research, but most can be adopted for research in other organisms.

Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3’-end formation in plants.

Editors and Affiliations

  • Department of Plant and Soil Sciences, University of Kentucky, Lexington, USA

    Arthur G. Hunt

  • Department of Biology, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Miami University, Oxford, OH, USA and Xiamen University, Xiamen, Fujian, China

    Qingshun Quinn Li

Bibliographic Information

  • Book Title: Polyadenylation in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Arthur G. Hunt, Qingshun Quinn Li

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2175-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2174-4Published: 09 December 2014

  • Softcover ISBN: 978-1-4939-4668-6Published: 22 September 2016

  • eBook ISBN: 978-1-4939-2175-1Published: 08 December 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 225

  • Number of Illustrations: 11 b/w illustrations, 23 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Gene Function

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access