Abstract
Because they are firmly held in place, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cornell et al. Nature 387(6633): 580–583, 1997). Here we describe the procedures required to assemble and test tethered lipid bilayers that can incorporate various lipid species, peptides, and ion channel proteins.
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References
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Acknowledgments
This work was supported by the Australian Research Council, the National Health and Medical Research Council of Australia (Grant 1047980). We declare that Bruce Cornell is a shareholder, and Sonia Carne is an employee, of SDx Tethered Membranes Pty Ltd.
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Cranfield, C., Carne, S., Martinac, B., Cornell, B. (2015). The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs). In: Owen, D. (eds) Methods in Membrane Lipids. Methods in Molecular Biology, vol 1232. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1752-5_4
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DOI: https://doi.org/10.1007/978-1-4939-1752-5_4
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